Direct-method structure determination of the native azurin II protein using one-wavelength anomalous scattering data
文献类型：期刊论文
| 作者 | Zheng, XF ; Fan, HF ; Hao, Q ; Dodd, FE ; Hasnain, SS |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
 |
| 出版日期 | 1996 |
| 卷号 | 52页码:937 |
| 关键词 | COMBINING DIRECT METHODS MACROMOLECULAR CRYSTALLOGRAPHY ALCALIGENES-XYLOSOXIDANS ISOMORPHOUS REPLACEMENT CRYSTAL-STRUCTURE RESOLUTION DIFFRACTION REFINEMENT POSITIONS MODELS |
| ISSN号 | 0907-4449 |
| 通讯作者 | Zheng, XF: CHINESE ACAD SCI,INST PHYS,POB 603,BEIJING 100080,PEOPLES R CHINA. |
| 中文摘要 | The one-wavelength anomalous scattering (GAS) X-ray diffraction data of azurin II, a copper-containing protein from Alcaligenes xylosoxidans were collected at the Photon Factory, Japan at a 'routine' wavelength of 0.97 Angstrom. The structure had been originally solved by the molecular-replacement method [Dodd, Hasnain, Abraham, Eady & Smith (1995). Acta Cryst. D51, 1052-1064]. As a technique of ab initio structure determination the direct method [Fan, Hao, Gu, Qian, Zheng & Ke (1990). Acta Cryst. A46, 935-939] was attempted to break the phase ambiguity intrinsic to OAS data. The phases were then improved using the solvent-flattening method. The final electron-density map clearly shows most C alpha positions and many side chains and it is traceable without prior knowledge of the structure. It is concluded that the direct method is capable of phasing anomalous scattering data collected at one wavelength from moderate-sized native proteins (M(w) similar or equal to 20 kDa) which contain copper or atoms with a similar scattering power. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/36107]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zheng, XF,Fan, HF,Hao, Q,et al. Direct-method structure determination of the native azurin II protein using one-wavelength anomalous scattering data[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,1996,52:937. |
| APA | Zheng, XF,Fan, HF,Hao, Q,Dodd, FE,&Hasnain, SS.(1996).Direct-method structure determination of the native azurin II protein using one-wavelength anomalous scattering data.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,52,937. |
| MLA | Zheng, XF,et al."Direct-method structure determination of the native azurin II protein using one-wavelength anomalous scattering data".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 52(1996):937. |
入库方式： OAI收割
来源：物理研究所
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