Dynamics of strand passage catalyzed by topoisomerase II
文献类型：期刊论文
| 作者 | Xie, P |
| 刊名 | EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
 |
| 出版日期 | 2010 |
| 卷号 | 39期号:8页码:1251 |
| 关键词 | N-TERMINAL FRAGMENT GYRASE-B-PROTEIN STEADY-STATE ANALYSIS COLI DNA-GYRASE ATP HYDROLYSIS STRUCTURAL MECHANISM NUCLEOTIDE-BINDING CRYSTAL-STRUCTURE 2-GATE MECHANISM KINESIN MOTORS |
| ISSN号 | 0175-7571 |
| 通讯作者 | Xie, P: Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | DNA topoisomerase II is a homodimeric molecular machine that uses ATP hydrolysis to untangle DNA by passing one double-stranded DNA duplex (T-segment) through another double-stranded duplex (G-segment). However, despite extensive studies, the dynamics of ATP-dependent T-transport is still not very clear. Here, based on the proposal that transport of the T-segment through the transiently cleaved G-segment and the opened C-gate of the enzyme is via a free diffusion mechanism, the dynamics of T-transport are studied theoretically. Our results show that, to complete passage of the strand with nearly 100% efficiency, the C-gate is required to open by a width that is only slightly larger than the width of DNA duplex and for a time shorter than 100 mu s in the presence of several k (B) T binding affinities of the T-segment for the B' domains. The results are implied by our understanding of the opening and closing dynamics of the C-gate. Moreover, the dependence of chemomechanical coupling efficiency on degrees of DNA supercoiling by gyrases can also be explained by using our results. On the basis of these theoretical results and previous experimental data, a modified two-gate model for chemomechanical coupling of the topoisomerase II enzyme is proposed. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [10974248] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/36306]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xie, P. Dynamics of strand passage catalyzed by topoisomerase II[J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,2010,39(8):1251. |
| APA | Xie, P.(2010).Dynamics of strand passage catalyzed by topoisomerase II.EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,39(8),1251. |
| MLA | Xie, P."Dynamics of strand passage catalyzed by topoisomerase II".EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 39.8(2010):1251. |
入库方式： OAI收割
来源：物理研究所
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