Effects of Paclitaxel on EGFR Endocytic Trafficking Revealed Using Quantum Dot Tracking in Single Cells
文献类型：期刊论文
| 作者 | Li, H ; Duan, ZW ; Xie, P ; Liu, YR ; Wang, WC ; Dou, SX ; Wang, PY |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:9 |
| 关键词 | EPIDERMAL GROWTH-FACTOR CLATHRIN-MEDIATED ENDOCYTOSIS RECEPTOR TYROSINE KINASES LIVING CELLS LIVE CELLS INTERMEDIATE-FILAMENTS AXONAL-TRANSPORT ERBB RECEPTORS A431 CELLS TAXOL |
| ISSN号 | 1932-6203 |
| 通讯作者 | Wang, PY: Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, Beijing Natl Lab Condensed Matter Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | Paclitaxel (PTX), a chemotherapeutic drug, affects microtubule dynamics and influences endocytic trafficking. However, the mechanism and the dynamics of altered endocytic trafficking by paclitaxel treatment in single living cells still remain elusive. By labeling quantum dots (QDs) to the epidermal growth factor (EGF), we continuously tracked the endocytosis and post-endocytic trafficking of EGF receptors (EGFRs) in A549 cells for a long time interval. A single-cell analysis method was introduced to quantitatively study the dynamics of endocytic trafficking. Compared with the control cells, the velocity of directed motion was reduced by 30% due to the suppression of high speed movements of EGF-QDs along the microtubules in PTX-treated cells. The endocytic trafficking in PTX-treated cells was mainly via super-diffusive mode of motion, whereas in control cells, it was mostly via sub-diffusive mode of motion. Moreover, PTX shortened endosomal trafficking and prevented EGF-QDs from moving to the perinuclear area via the rapid delivery of EGF-QDs into the peripheral lysosomes. The present study may shed light on the mechanism of the effect of PTX on the treatment of lung cancer. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [10834014]; National Basic Research Program of China (973 Program) [2009CB930704] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/36783]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, H,Duan, ZW,Xie, P,et al. Effects of Paclitaxel on EGFR Endocytic Trafficking Revealed Using Quantum Dot Tracking in Single Cells[J]. PLOS ONE,2012,7(9). |
| APA | Li, H.,Duan, ZW.,Xie, P.,Liu, YR.,Wang, WC.,...&Wang, PY.(2012).Effects of Paclitaxel on EGFR Endocytic Trafficking Revealed Using Quantum Dot Tracking in Single Cells.PLOS ONE,7(9). |
| MLA | Li, H,et al."Effects of Paclitaxel on EGFR Endocytic Trafficking Revealed Using Quantum Dot Tracking in Single Cells".PLOS ONE 7.9(2012). |
入库方式： OAI收割
来源：物理研究所
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