Energy landscape of aptamer/protein complexes studied by single-molecule force spectroscopy
文献类型：期刊论文
| 作者 | Yu, JP ; Jiang, YX ; Ma, XY ; Lin, Y ; Fang, XH |
| 刊名 | CHEMISTRY-AN ASIAN JOURNAL
 |
| 出版日期 | 2007 |
| 卷号 | 2期号:2页码:284 |
| 关键词 | DNA RESPONSIVE ELEMENT UNBINDING FORCES MICROSCOPY ADHESION BONDS APTAMERS PROTEIN DISSOCIATION RECOGNITION DEPENDENCE |
| ISSN号 | 1861-4728 |
| 通讯作者 | Fang, XH: Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China. |
| 中文摘要 | Aptamers are single-stranded nucleic acid molecules selected in vitro to bind to a variety of target molecules. Aptamers bound to proteins are emerging as a new class of molecules that rival commonly used antibodies in both therapeutic and diagnostic applications. With the increasing application of aptamers as molecular probes for protein recognition, it is important to understand the molecular mechanism of aptamer-protein interaction. Recently, we developed a method of using atomic force microscopy (AFM) to study the single-molecule rupture force of aptamer/protein complexes. In this work, we investigate further the unbinding dynamics of aptamer/protein complexes and their dissociation-energy landscape by AFM. The dependence of single-molecule force on the AFM loading rate was plotted for three aptamer/protein complexes and their dissociation rate constants, and other parameters characterizing their dissociation pathways were obtained. Furthermore, the single-molecule force spectra of three aptamer/protein complexes were compared to those of the corresponding antibody/protein complexes in the same loading-rate range. The results revealed two activation barriers and one intermediate state in the unbinding process of aptamer/protein complexes, which is different from the energy landscape of antibody/protein complexes. The results provide new information for the study of aptamer-protein interaction at the molecular level. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/37307]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yu, JP,Jiang, YX,Ma, XY,et al. Energy landscape of aptamer/protein complexes studied by single-molecule force spectroscopy[J]. CHEMISTRY-AN ASIAN JOURNAL,2007,2(2):284. |
| APA | Yu, JP,Jiang, YX,Ma, XY,Lin, Y,&Fang, XH.(2007).Energy landscape of aptamer/protein complexes studied by single-molecule force spectroscopy.CHEMISTRY-AN ASIAN JOURNAL,2(2),284. |
| MLA | Yu, JP,et al."Energy landscape of aptamer/protein complexes studied by single-molecule force spectroscopy".CHEMISTRY-AN ASIAN JOURNAL 2.2(2007):284. |
入库方式： OAI收割
来源：物理研究所
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