Enhancement of nanofibrous scaffold of multiwalled carbon nanotubes/polyurethane composite to the fibroblasts growth and biosynthesis
文献类型：期刊论文
| 作者 | Meng, J ; Kong, H ; Han, ZZ ; Wang, CY ; Zhu, GJ ; Xie, SS ; Xu, HY |
| 刊名 | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
 |
| 出版日期 | 2009 |
| 卷号 | 88A期号:1页码:105 |
| 关键词 | NORMAL HUMAN KERATINOCYTES MESENCHYMAL STEM-CELLS IN-VITRO EXTRACELLULAR-MATRIX BLOOD COMPATIBILITY PROTEIN ADSORPTION CELLULAR-RESPONSE NANOTUBES COLLAGEN FUNCTIONALIZATION |
| ISSN号 | 1549-3296 |
| 通讯作者 | Xu, HY: Chinese Acad Sci, Inst Basic Med Sci, Dept Biomed Engn, Beijing 100005, Peoples R China. |
| 中文摘要 | In this work, the effect of nanofibrous structure and multiwalled carbon nanotubes (MWNTs) incorporation in the polyurethane (PU) on the fibroblasts growth behavior was studied. The nanofibrous scaffold of multiwalled carbon nanotubes and polyurethane composite (MWNT/PU) with an average fiber diameter of 300-500 nm was fabricated by electrospinning technique. The nanofibrous scaffold of PU, smooth film of PU, and MWNT/PU were also prepared as controls. Cell viability assay, laser confocal microscopy, and scanning electron microscopy were applied to evaluate cell adhesion, proliferation, and cytoskeletal development on the scaffolds, respectively. Cell-released protein was analyzed by Bradford protein assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), mass spectrometry, and transwell assay, respectively. Experimental results demonstrated that the scaffold with nanofibrous structure and MWNTs incorporation exhibited highest enhancement not only to the cell adhesion and proliferation but also to the cell migration and aggregation. Besides, cells cultured on the nanofibrous, scaffold of MWNT/PU released the largest amount of proteins including collagen in comparison with those on the other substrates. Hence, the nanofibrous architecture and MWNTs incorporation provided favorite interactions to the cells, which implied the application potentials of the nanofibrous composite for tissue repair and regeneration. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 88A: 105-116, 2009 |
| 收录类别 | SCI |
| 资助信息 | Natural Science Foundation of China [90606018]; National Center for Nanoscience and Technology, China |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/37422]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Meng, J,Kong, H,Han, ZZ,et al. Enhancement of nanofibrous scaffold of multiwalled carbon nanotubes/polyurethane composite to the fibroblasts growth and biosynthesis[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2009,88A(1):105. |
| APA | Meng, J.,Kong, H.,Han, ZZ.,Wang, CY.,Zhu, GJ.,...&Xu, HY.(2009).Enhancement of nanofibrous scaffold of multiwalled carbon nanotubes/polyurethane composite to the fibroblasts growth and biosynthesis.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,88A(1),105. |
| MLA | Meng, J,et al."Enhancement of nanofibrous scaffold of multiwalled carbon nanotubes/polyurethane composite to the fibroblasts growth and biosynthesis".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 88A.1(2009):105. |
入库方式： OAI收割
来源：物理研究所
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