Escherichia coli RecQ is a rapid, efficient, and monomeric helicase
文献类型：期刊论文
| 作者 | Zhang, XD ; Dou, SX ; Xie, P ; Hu, JS ; Wang, PY ; Xi, XG |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2006 |
| 卷号 | 281期号:18页码:12655 |
| 关键词 | SINGLE-STRANDED-DNA BACTERIOPHAGE-T4 DDA HELICASE TERMINATION FACTOR-RHO C VIRUS HELICASE CRYSTAL-STRUCTURE PCRA HELICASE REP HELICASE STEP-SIZE FLUORESCENCE POLARIZATION HEXAMERIC HELICASES |
| ISSN号 | 0021-9258 |
| 通讯作者 | Wang, PY: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Lab Soft Matter Phys, Beijing 100080, Peoples R China. |
| 中文摘要 | RecQ family helicases play a key role in chromosome maintenance. Despite extensive biochemical, biophysical, and structural studies, the mechanism by which helicase unwinds double-stranded DNA remains to be elucidated. Using a wide array of biochemical and biophysical approaches, we have previously shown that the Escherichia coli RecQ helicase functions as a monomer. In this study, we have further characterized the kinetic mechanism of the RecQ-catalyzed unwinding of duplex DNA using the fluorometric stopped-flow method based on fluorescence resonance energy transfer. Our results show that RecQ helicase binds preferentially to 3'-flanking duplex DNA. Under the pre-steady-state conditions, the burst amplitude reveals a 1: 1 ratio between RecQ and DNA substrate, suggesting that an active monomeric form of RecQ helicase is involved in the catalysis. Under the single-turnover conditions, the RecQ-catalyzed unwinding is independent of the 3'-tail length, indicating that functional interactions between RecQ molecules are not implicated in the DNA unwinding. It was further determined that RecQ unwinds DNA rapidly with a step size of 4 bp and a rate of similar to 21 steps/s. These kinetic results not only further support our previous conclusion that E. coli RecQ functions as a monomer but also suggest that some of the Superfamily 2 helicases may function through an "inchworm" mechanism. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/37575]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang, XD,Dou, SX,Xie, P,et al. Escherichia coli RecQ is a rapid, efficient, and monomeric helicase[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2006,281(18):12655. |
| APA | Zhang, XD,Dou, SX,Xie, P,Hu, JS,Wang, PY,&Xi, XG.(2006).Escherichia coli RecQ is a rapid, efficient, and monomeric helicase.JOURNAL OF BIOLOGICAL CHEMISTRY,281(18),12655. |
| MLA | Zhang, XD,et al."Escherichia coli RecQ is a rapid, efficient, and monomeric helicase".JOURNAL OF BIOLOGICAL CHEMISTRY 281.18(2006):12655. |
入库方式： OAI收割
来源：物理研究所
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