Expression, purification, crystallization and preliminary X-ray diffraction analysis of grass carp beta(2)-microglobulin
文献类型：期刊论文
| 作者 | Chen, WH ; Chu, FL ; Peng, H ; Zhang, JH ; Qi, JX ; Jiang, F ; Xia, C ; Gao, F |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
 |
| 出版日期 | 2008 |
| 卷号 | 64页码:200 |
| ISSN号 | 1744-3091 |
| 中文摘要 | beta(2)-Microglobulin (beta(2)m) is an essential subunit of MHC I molecules; it stabilizes the structure of MHC I and plays a pivotal role in coreceptor recognition. To date, structures of beta(2)m have been solved for three different mammals: human, mouse and cattle. In order to illuminate the molecular evolutionary origin of beta(2)m, an understanding of its structure in lower vertebrates becomes important. Here, grass carp (Ctenopharyngodon idellus) beta(2)m (Ctid-beta(2)m) was expressed, purified and crystallized. Diffraction data were collected to a resolution of 2.5 angstrom. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.72, b = 40.65, c = 71.12 angstrom. The Matthews coefficient and the solvent content were calculated to be 2.56 angstrom Da(-1) and 52.07%, respectively, for one molecule per asymmetric unit. The structure has been solved by molecular replacement using monomeric human beta(2)m as a model. |
| 收录类别 | SCI |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/37838]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen, WH,Chu, FL,Peng, H,et al. Expression, purification, crystallization and preliminary X-ray diffraction analysis of grass carp beta(2)-microglobulin[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2008,64:200. |
| APA | Chen, WH.,Chu, FL.,Peng, H.,Zhang, JH.,Qi, JX.,...&Gao, F.(2008).Expression, purification, crystallization and preliminary X-ray diffraction analysis of grass carp beta(2)-microglobulin.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,64,200. |
| MLA | Chen, WH,et al."Expression, purification, crystallization and preliminary X-ray diffraction analysis of grass carp beta(2)-microglobulin".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 64(2008):200. |
入库方式： OAI收割
来源：物理研究所
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