Fluorometric assays for characterizing DNA helicases
文献类型：期刊论文
| 作者 | Dou, SX ; Xi, XG |
| 刊名 | METHODS
 |
| 出版日期 | 2010 |
| 卷号 | 51期号:3页码:295 |
| 关键词 | STRAND-ANNEALING ACTIVITIES HEPATITIS-C VIRUS HUMAN RECQ5-BETA BINDING FLUORESCENCE PROTEIN PROCESSIVITY TRANSLOCASES MECHANISMS ENZYME |
| ISSN号 | 1046-2023 |
| 通讯作者 | Dou, SX: Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | DNA helicases belong to an important class of motor proteins and are involved in almost all aspects of DNA metabolism. They hydrolyze NTP to translocate along ssDNA and unwind dsDNA by relying on chemical to physical energy transfer processes that are achieved via nucleotide-state-dependent conformational changes. For understanding the mechanisms by which helicases unwind DNA as well as their cellular functions, various properties of helicases need to be characterized. For these purposes, many assays have been developed, among which fluorometric assays are in the majority. Fluorometric assays are generally simple, direct and convenient to perform. Here we introduce several frequently used fluorometric assays for determining the basic properties of DNA helicases such as equilibrium ATP and DNA binding, kinetics of dissociation from DNA substrate and kinetics of DNA unwinding. Problems that may be encountered in experiments and possible ways to circumvent them are discussed. (C) 2010 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China; 973 Project; Institut Curie and the Centre National de la Recherche Scientifique (CNRS) |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/38303]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Dou, SX,Xi, XG. Fluorometric assays for characterizing DNA helicases[J]. METHODS,2010,51(3):295. |
| APA | Dou, SX,&Xi, XG.(2010).Fluorometric assays for characterizing DNA helicases.METHODS,51(3),295. |
| MLA | Dou, SX,et al."Fluorometric assays for characterizing DNA helicases".METHODS 51.3(2010):295. |
入库方式： OAI收割
来源：物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。