Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure
文献类型：期刊论文
| 作者 | Wang, XL ; Zhang, XH ; Cao, MW ; Zheng, HZ ; Xiao, B ; Wang, YL ; Li, M |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2009 |
| 卷号 | 113期号:8页码:2328 |
| 关键词 | LAMBDA-PHAGE DNA CATIONIC SURFACTANT MOLECULES NANOPARTICLES TRANSFECTION NUCLEATION COMPACTION ELASTICITY TRANSITION DYNAMICS |
| ISSN号 | 1520-6106 |
| 通讯作者 | Li, M: Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Using a magnetic tweezers (MT) apparatus and an atomic force microscope (AFM), we studied the condensation of DNA induced by the cationic gemini surfactant hexyl-alpha,omega-bisdodecyidimethylammonium bromide (C(12)C(6)C(12)Br(2)). Stepwise condensation events were found for forces from 0.5 to 4 pN, with a decrease in DNA extension by similar to 100 nm in each condensation event. Applied stretching forces larger than 6 pN were found to be able to decondense the condensates in a similar intermittent stepwise manner, but with similar to 60 nm of DNA released in each decondensation event. These observations are consistent with AFM images that show beadlike structures on DNA. The results lead to a model in which, during condensation, independent beadlike structures are initially formed on DNA, and as the local density of the beads increases, they compact into higher-order structures while maintaining their independence. The condensation process is different from any previously reported condensation process, but it is somewhat similar to that of chromatin assembly. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/38606]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, XL,Zhang, XH,Cao, MW,et al. Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(8):2328. |
| APA | Wang, XL.,Zhang, XH.,Cao, MW.,Zheng, HZ.,Xiao, B.,...&Li, M.(2009).Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure.JOURNAL OF PHYSICAL CHEMISTRY B,113(8),2328. |
| MLA | Wang, XL,et al."Gemini Surfactant-Induced DNA Condensation into a Beadlike Structure".JOURNAL OF PHYSICAL CHEMISTRY B 113.8(2009):2328. |
入库方式： OAI收割
来源：物理研究所
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