High contrast femtosecond laser-driven intense hard X-ray source for imaging application
文献类型：期刊论文
| 作者 | Chen, LM ; Wang, WM ; Kando, M ; Hudson, LT ; Liu, F ; Lin, XX ; Ma, JL ; Li, YT ; Bulanov, SV ; Tajima, T ; Kato, Y ; Sheng, ZM ; Zhang, J |
| 刊名 | NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
 |
| 出版日期 | 2010 |
| 卷号 | 619期号:1-3页码:128 |
| 关键词 | PLASMA INTERACTIONS ABSORPTION EMISSION PULSES ELECTRON |
| ISSN号 | 0168-9002 |
| 通讯作者 | Chen, LM: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | DNA helicases belong to an important class of motor proteins and are involved in almost all aspects of DNA metabolism. They hydrolyze NTP to translocate along ssDNA and unwind dsDNA by relying on chemical to physical energy transfer processes that are achieved via nucleotide-state-dependent conformational changes. For understanding the mechanisms by which helicases unwind DNA as well as their cellular functions, various properties of helicases need to be characterized. For these purposes, many assays have been developed, among which fluorometric assays are in the majority. Fluorometric assays are generally simple, direct and convenient to perform. Here we introduce several frequently used fluorometric assays for determining the basic properties of DNA helicases such as equilibrium ATP and DNA binding, kinetics of dissociation from DNA substrate and kinetics of DNA unwinding. Problems that may be encountered in experiments and possible ways to circumvent them are discussed. (C) 2010 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/39174]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen, LM,Wang, WM,Kando, M,et al. High contrast femtosecond laser-driven intense hard X-ray source for imaging application[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,2010,619(1-3):128. |
| APA | Chen, LM.,Wang, WM.,Kando, M.,Hudson, LT.,Liu, F.,...&Zhang, J.(2010).High contrast femtosecond laser-driven intense hard X-ray source for imaging application.NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,619(1-3),128. |
| MLA | Chen, LM,et al."High contrast femtosecond laser-driven intense hard X-ray source for imaging application".NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT 619.1-3(2010):128. |
入库方式： OAI收割
来源：物理研究所
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