Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditions
文献类型：期刊论文
| 作者 | Qu, Y ; Bi, LJ ; Ji, XL ; Deng, ZL ; Zhang, HT ; Yan, YF ; Wang, M ; Li, AQ ; Huang, XX ; Yang, RF ; Han, YP |
| 刊名 | FUTURE MICROBIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:4页码:535 |
| 关键词 | ESCHERICHIA-COLI REGULATORY RNAS ANTISENSE RNAS NONCODING RNAS VIRULENCE BACTERIA HFQ DISCOVERY PLAGUE SMPB |
| ISSN号 | 1746-0913 |
| 通讯作者 | Han, YP: Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China. |
| 中文摘要 | Aims: sRNA regulation is supposedly involved in the stress response of a pathogen during infection. Yersinia pest's, the etiologic agent of plague, must encounter temperature and microenvironment changes, given its lifestyle, Here, we used the cDNA cloning approach to discover full-length sRNA candidates that are highly expressed in Y. pestis under five different growth conditions. Materials & methods: The cDNA cloning approach was improved by combining the traditional cDNA library construction with the prevalent rapid amplification of cDNA ends and RNA size selection techniques. Results: In total, 43 RNA species, including six previously annotated sRNAs, were identified. Of these, 25 sRNAs were encoded on the antisense strand of the annotated genes. Interestingly, two of these sRNAs were found on the complementary strand of noncoding RNAs. In addition, eight novel sRNAs encoded in the intergenic regions were also revealed. Ten sRNA candidates chosen for the northern blot analysis were successfully defected. Analysis of the expression patterns of 29 candidate sRNAs showed that 24 sRNAs are highly abundant in Y pestis upon entry into the stationary growth phase. Conclusion: Our preliminary attempt at screening the novel sRNA candidates will lay the foundation for understanding the roles of sRNAs in Y. pestis physiology and pathogenesis. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30971629, 31171248, 30930001] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/39581]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qu, Y,Bi, LJ,Ji, XL,et al. Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditions[J]. FUTURE MICROBIOLOGY,2012,7(4):535. |
| APA | Qu, Y.,Bi, LJ.,Ji, XL.,Deng, ZL.,Zhang, HT.,...&Han, YP.(2012).Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditions.FUTURE MICROBIOLOGY,7(4),535. |
| MLA | Qu, Y,et al."Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditions".FUTURE MICROBIOLOGY 7.4(2012):535. |
入库方式： OAI收割
来源：物理研究所
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