Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding
文献类型：期刊论文
| 作者 | Sun, B ; Wei, KJ ; Zhang, B ; Zhang, XH ; Dou, SX ; Li, M ; Xi, XG |
| 刊名 | EMBO JOURNAL
 |
| 出版日期 | 2008 |
| 卷号 | 27期号:24页码:3279 |
| 关键词 | SINGLE-STRANDED-DNA REP HELICASE TRANSLOCATION MOLECULE PROTEIN TIME ATP |
| ISSN号 | 0261-4189 |
| 通讯作者 | Li, M: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Escherichia coli UvrD is a non-ring-shaped model helicase, displaying a 3'-5' polarity in DNA unwinding. Using a transverse magnetic tweezer and DNA hairpins, we measured the unwinding kinetics of UvrD at various DNA-destabilizing forces. The multiform patterns of unwinding bursts and the distributions of the off-times favour the mechanism that UvrD unwinds DNA as a dimer. The two subunits of the dimer coordinate to unwind DNA processively. They can jointly switch strands and translocate backwards on the other strand to allow slow (similar to 40 bp/s) rewinding, or unbind simultaneously to allow quick rehybridization. Partial dissociation of the dimer results in pauses in the middle of the unwinding or increases the translocation rate from similar to 40 to similar to 150 nt/s in the middle of the rewinding. Moreover, the unwinding rate was surprisingly found to decrease from similar to 45 to similar to 10 bp/s when the force is increased from 2 to 12 pN. The results lead to a strained-inchworm mechanism in which a conformational change that bends and tenses the ssDNA is required to activate the dimer. |
| 收录类别 | SCI |
| 资助信息 | National Key Basic Research Program of China [2006CB910302]; National Natural Science Foundation of China [30870590]; Centre National de la Recherche Scientifique and Institut Curie |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/39636]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun, B,Wei, KJ,Zhang, B,et al. Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding[J]. EMBO JOURNAL,2008,27(24):3279. |
| APA | Sun, B.,Wei, KJ.,Zhang, B.,Zhang, XH.,Dou, SX.,...&Xi, XG.(2008).Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding.EMBO JOURNAL,27(24),3279. |
| MLA | Sun, B,et al."Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding".EMBO JOURNAL 27.24(2008):3279. |
入库方式： OAI收割
来源：物理研究所
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