Ionic effect on combing of single DNA molecules. and observation of their force-induced melting by fluorescence microscopy
文献类型：期刊论文
| 作者 | Liu, YY ; Wang, PY ; Dou, SX ; Wang, WC ; Xie, P ; Yin, HW ; Zhang, XD |
| 刊名 | JOURNAL OF CHEMICAL PHYSICS
 |
| 出版日期 | 2004 |
| 卷号 | 121期号:9页码:4302 |
| 关键词 | DOUBLE-STRANDED DNA OVERSTRETCHING TRANSITION DOUBLE HELIX ELASTICITY BINDING SPECTROSCOPY MENISCUS MOTION YOYO |
| ISSN号 | 0021-9606 |
| 通讯作者 | Wang, PY: Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, we observed that the length of X-DNA molecules was extended to about 1.6 times their contour length (unextended length, 16.2 mum) by the combing method on hydrophobic polymethylmetacrylate coated surfaces. The effects of sodium and magnesium ions and pH of the DNA solution were investigated. Interestingly, we observed force-induced melting of single DNA molecules. (C) 2004 American Institute of Physics. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/40377]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, YY,Wang, PY,Dou, SX,et al. Ionic effect on combing of single DNA molecules. and observation of their force-induced melting by fluorescence microscopy[J]. JOURNAL OF CHEMICAL PHYSICS,2004,121(9):4302. |
| APA | Liu, YY.,Wang, PY.,Dou, SX.,Wang, WC.,Xie, P.,...&Zhang, XD.(2004).Ionic effect on combing of single DNA molecules. and observation of their force-induced melting by fluorescence microscopy.JOURNAL OF CHEMICAL PHYSICS,121(9),4302. |
| MLA | Liu, YY,et al."Ionic effect on combing of single DNA molecules. and observation of their force-induced melting by fluorescence microscopy".JOURNAL OF CHEMICAL PHYSICS 121.9(2004):4302. |
入库方式： OAI收割
来源：物理研究所
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