Kinetic Mechanism of DNA Unwinding by the BLM Helicase Core and Molecular Basis for Its Low Processivity
文献类型：期刊论文
| 作者 | Yang, Y ; Dou, SX ; Xu, YN ; Bazeille, N ; Wang, PY ; Rigolet, P ; Xu, HQ ; Xi, XG |
| 刊名 | BIOCHEMISTRY
 |
| 出版日期 | 2010 |
| 卷号 | 49期号:4页码:656 |
| 关键词 | SINGLE-STRANDED-DNA ESCHERICHIA-COLI RECQ BLOOMS-SYNDROME HELICASE SYNDROME GENE-PRODUCT C VIRUS HELICASE SYNDROME PROTEIN STEP-SIZE HOMOLOGOUS RECOMBINATION MONOMERIC HELICASE HEXAMERIC HELICASE |
| ISSN号 | 0006-2960 |
| 通讯作者 | Dou, SX: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Bloom's syndrome (BS) is a rare human autosomal recessive disorder characterized by a strong predisposition to a wide range of cancers commonly affecting the general population. Understanding the functioning mechanism of the BLM protein may provide the opportunity to develop new effective therapy strategies. In this work, we studied the DNA unwinding kinetic mechanism of the helicase core of the BLM protein using various stopped-now assays. We show that the helicase core of BLM unwinds duplex DNA as monomers even under conditions strongly favoring oligomerization. An unwinding rate of similar to 20 steps per second and a step size of 1 bp have been determined. We have observed that the helicase has a very low processivity. From dissociation and inhibition experiments, we have found that during its ATP hydrolysis cycle in DNA unwinding the helicase tends to dissociate from the DNA substrate in the ADP state. The experimental results imply that the BLM helicase core may unwind duplex DNA in an inchworm manner. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China; 973 Project; Shanghai Municipal Science and Technology Commission [07DZ22940]; Shanghai Municipal Wildlife Administration [SBHZ2006_01]; Institut Curie; Centre National de la Recherche Scientifique (CNRS) |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/40459]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang, Y,Dou, SX,Xu, YN,et al. Kinetic Mechanism of DNA Unwinding by the BLM Helicase Core and Molecular Basis for Its Low Processivity[J]. BIOCHEMISTRY,2010,49(4):656. |
| APA | Yang, Y.,Dou, SX.,Xu, YN.,Bazeille, N.,Wang, PY.,...&Xi, XG.(2010).Kinetic Mechanism of DNA Unwinding by the BLM Helicase Core and Molecular Basis for Its Low Processivity.BIOCHEMISTRY,49(4),656. |
| MLA | Yang, Y,et al."Kinetic Mechanism of DNA Unwinding by the BLM Helicase Core and Molecular Basis for Its Low Processivity".BIOCHEMISTRY 49.4(2010):656. |
入库方式： OAI收割
来源：物理研究所
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