Kinetic study of the DNA annealing properties of RECQ5 beta helicase
文献类型：期刊论文
| 作者 | Ding, XY ; Xu, YN ; Li, W ; Wang, PY ; Xi, XG ; Dou, SX |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2012 |
| 卷号 | 57期号:11页码:1280 |
| 关键词 | ESCHERICHIA-COLI RECQ HELICASES SYNDROME GENE CLONING FAMILY MECHANISM PROTEIN GENOME ROLES |
| ISSN号 | 1001-6538 |
| 通讯作者 | Dou, SX: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | RecQ family helicases are critical for maintaining genomic integrity. Many RecQ family helicases not only unwind duplex, and other more complicated DNA structures, but also possess, interestingly, DNA annealing (strand pairing) activity. Here, we systematically investigated the DNA annealing properties of RECQ5 beta by measuring DNA annealing kinetics, equilibrium DNA binding, and kinetics of dissociation from ssDNA. RECQ5 beta catalyzed DNA annealing most efficiently when the enzyme molecules covered approximately 40%-50% of the DNA strand, in the absence or presence of different nucleotide cofactors (AMPPNP, ATP gamma S, or ADP) under our buffer conditions. A comparative study with RECQ5 beta(1-662) confirmed that the C-terminal region of RECQ5 beta was essential for its high DNA annealing activity. These results contribute to our understanding of the mechanism of DNA annealing catalyzed by RecQ family helicases. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [10834014]; National Basic Research Program of China [2009CB930704] |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/40463]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ding, XY,Xu, YN,Li, W,et al. Kinetic study of the DNA annealing properties of RECQ5 beta helicase[J]. CHINESE SCIENCE BULLETIN,2012,57(11):1280. |
| APA | Ding, XY,Xu, YN,Li, W,Wang, PY,Xi, XG,&Dou, SX.(2012).Kinetic study of the DNA annealing properties of RECQ5 beta helicase.CHINESE SCIENCE BULLETIN,57(11),1280. |
| MLA | Ding, XY,et al."Kinetic study of the DNA annealing properties of RECQ5 beta helicase".CHINESE SCIENCE BULLETIN 57.11(2012):1280. |
入库方式： OAI收割
来源：物理研究所
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