Label-free and real-time detection of antigen-antibody interactions by Oblique-incidence Reflectivity Difference (OIRD) method
文献类型：期刊论文
| 作者 | He, LP ; Sun, Y ; Dai, J ; Wang, JY ; Lu, HB ; Wang, SF ; Jin, KJ ; Zhou, YL ; Yang, GZ |
| 刊名 | SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
 |
| 出版日期 | 2012 |
| 卷号 | 55期号:9页码:1585 |
| 关键词 | PROTEIN MICROARRAYS |
| ISSN号 | 1674-7348 |
| 通讯作者 | Lu, HB: Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | We label-free and real-time detected three interaction processes of antigen-antibodies, Human Immunoglobulin G (IgG), Rabbit IgG, and Mouse IgG as the targets, and Goat Anti-human IgG, Goat Anti-rabbit IgG, and Goat Anti-mouse IgG as the probe, by the Oblique-incidence Reflectivity Difference (OIRD) method. The interaction dynamic curves of the OIRD signal, corresponding to the interaction processes of antigen-antibodies, are generated. The reaction times from beginning to equilibrium state are about 1800, 900, and 1200 s for Human IgG, Rabbit IgG, and Mouse IgG, respectively. The experimental results demonstrate that the OIRD method not only can distinguish biomolecular interactions, but also can be used in real-time detection of interactions and dynamic processes of biomolecules. |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2007CB935701] |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/40529]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | He, LP,Sun, Y,Dai, J,et al. Label-free and real-time detection of antigen-antibody interactions by Oblique-incidence Reflectivity Difference (OIRD) method[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2012,55(9):1585. |
| APA | He, LP.,Sun, Y.,Dai, J.,Wang, JY.,Lu, HB.,...&Yang, GZ.(2012).Label-free and real-time detection of antigen-antibody interactions by Oblique-incidence Reflectivity Difference (OIRD) method.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,55(9),1585. |
| MLA | He, LP,et al."Label-free and real-time detection of antigen-antibody interactions by Oblique-incidence Reflectivity Difference (OIRD) method".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 55.9(2012):1585. |
入库方式： OAI收割
来源：物理研究所
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