Method used to measure interaction of proteins with dual-beam optical tweezers
文献类型：期刊论文
| 作者 | Qu, E ; Guo, HL ; Xu, CH ; Liu, CX ; Li, ZL ; Cheng, BY ; Zhang, DZ |
| 刊名 | JOURNAL OF BIOMEDICAL OPTICS
 |
| 出版日期 | 2006 |
| 卷号 | 11期号:6 |
| 关键词 | LIGAND-MEDIATED ADHESION LATEX SPHERES FORCE BOND CYTOSKELETON DISPLACEMENT FIBRONECTIN MOLECULE KINETICS FAILURE |
| ISSN号 | 1083-3668 |
| 通讯作者 | Qu, E: Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, POB 603-29, Beijing 100080, Peoples R China. |
| 中文摘要 | In the force measurement of protein-protein interaction, proteins are usually attached to microbeads, so the coated beads serve as both handles and force transducers. Due to the short interaction distance between proteins, the beads are usually close enough to each other. When dual-beam optical tweezers and quadrant photodiode detector are used to investigate the interaction of proteins, it is found that the signal of detected beads is greatly affected by adjacent beads. Analysis reveals that the contribution of two beads to the quadrant detector signal is independent. A method for extracting the real interaction signal from a disturbed one is presented. Based on this method, interaction between microtubules and AtMAP65-1 is measured. The results show that this method is useful for measuring short-distance interaction with the precision of piconewton and nanometer scales. (c) 2006 Society of Photo-Optical Instrumentation Engineers. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/41959]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qu, E,Guo, HL,Xu, CH,et al. Method used to measure interaction of proteins with dual-beam optical tweezers[J]. JOURNAL OF BIOMEDICAL OPTICS,2006,11(6). |
| APA | Qu, E.,Guo, HL.,Xu, CH.,Liu, CX.,Li, ZL.,...&Zhang, DZ.(2006).Method used to measure interaction of proteins with dual-beam optical tweezers.JOURNAL OF BIOMEDICAL OPTICS,11(6). |
| MLA | Qu, E,et al."Method used to measure interaction of proteins with dual-beam optical tweezers".JOURNAL OF BIOMEDICAL OPTICS 11.6(2006). |
入库方式： OAI收割
来源：物理研究所
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