Model for kinetics of wild-type and mutant kinesins
文献类型：期刊论文
| 作者 | Xie, P ; Dou, SX ; Wang, PY |
| 刊名 | BIOSYSTEMS
 |
| 出版日期 | 2006 |
| 卷号 | 84期号:1页码:24 |
| 关键词 | HAND-OVER-HAND 2 MOTOR DOMAINS MOLECULAR MOTORS BROWNIAN MOTOR CATALYTIC CORE ATP HYDROLYSIS FORCE CLAMP MECHANISM MOVEMENT MOTILITY |
| ISSN号 | 0303-2647 |
| 通讯作者 | Xie, P: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Lab Soft Matter Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | A hand-over-hand model is presented for the processive movement of two-headed kinesin based on previous structural and biochemical studies. In the model, the ATPase activities of the two heads are regulated by forces, both from internal elasticity and external load, exerted on their neck linkers. The results from the model show that the two heads may be partially coordinated in their ATPase cycles: in the case of backward load or low forward load, the ATPase cycles of its two heads are well coordinated, whereas in the case of high forward load, they are no longer well coordinated. The model gives results that show good quantitative agreement with both previous biochemical and mechanical experimental results such as the limping of homodimers and the dependences of mean velocity on [ATP] and on loads (both positive and negative). Furthermore, using the model we study the kinetics of a number of mutant kinesin homodimers and heterodimers, showing that the two heads' ATPase activities of some of these molecules are not well coordinated and they move processively with low mechanochemical coupling efficiencies even under no load. The theoretical results of ATPase rate per head, moving velocity, and stall force of the motors show good quantitative agreement with the experimental ones. The puzzling dynamic behaviours of mutant homodimeric and heterodimeric kinesins become understandable. (C) 2005 Elsevier Ireland Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/42205]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xie, P,Dou, SX,Wang, PY. Model for kinetics of wild-type and mutant kinesins[J]. BIOSYSTEMS,2006,84(1):24. |
| APA | Xie, P,Dou, SX,&Wang, PY.(2006).Model for kinetics of wild-type and mutant kinesins.BIOSYSTEMS,84(1),24. |
| MLA | Xie, P,et al."Model for kinetics of wild-type and mutant kinesins".BIOSYSTEMS 84.1(2006):24. |
入库方式： OAI收割
来源：物理研究所
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