Modeling translocation dynamics of strand displacement DNA synthesis by DNA polymerase I
文献类型：期刊论文
| 作者 | Xie, P |
| 刊名 | JOURNAL OF MOLECULAR MODELING
 |
| 出版日期 | 2012 |
| 卷号 | 18期号:5页码:1951 |
| 关键词 | CRYSTAL-STRUCTURE KLENOW FRAGMENT TEMPERATURE-DEPENDENCE REPLICATION COMPLEX EXONUCLEASE SITES STRUCTURAL BASIS SINGLE-MOLECULE RNA-POLYMERASE MECHANISM TRANSCRIPTION |
| ISSN号 | 1610-2940 |
| 通讯作者 | Xie, P: Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | A model is presented for the translocation dynamics of the strand displacement DNA synthesis by DNA polymerases such as polymerase I family. (i) The model gives an explanation to the experimental results which showed that the rate of strand displacement DNA synthesis is nearly consistent with that of single stranded primer extension synthesis, although the two are expected to have substantial differences in their energetics. (ii) During strand displacement DNA synthesis, the pausing at the specific sequence is considered to be due to an affinity of the fingers subdomain for the specific sequence of dsDNA downstream of the single strand. The theoretical results on the sequence-dependent pausing dynamics such as the mean pausing lifetimes and the distribution of the pausing lifetime are consistent with the experimental data. Moreover, predicted results are presented for the binding affinity of the fingers subdomain for the specific sequence of dsDNA and the dependence of the mean sequence-dependent pausing lifetime on the external force acting on the polymerase. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [10974248] |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/42215]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xie, P. Modeling translocation dynamics of strand displacement DNA synthesis by DNA polymerase I[J]. JOURNAL OF MOLECULAR MODELING,2012,18(5):1951. |
| APA | Xie, P.(2012).Modeling translocation dynamics of strand displacement DNA synthesis by DNA polymerase I.JOURNAL OF MOLECULAR MODELING,18(5),1951. |
| MLA | Xie, P."Modeling translocation dynamics of strand displacement DNA synthesis by DNA polymerase I".JOURNAL OF MOLECULAR MODELING 18.5(2012):1951. |
入库方式： OAI收割
来源：物理研究所
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