Modelling of a DNA packaging motor
文献类型：期刊论文
| 作者 | Qian, J ; Xie, P ; Xue, XG ; Wang, PY |
| 刊名 | CHINESE PHYSICS B
 |
| 出版日期 | 2009 |
| 卷号 | 18期号:11页码:4852 |
| 关键词 | BACTERIOPHAGE PHI-29 HOLLIDAY JUNCTIONS BRANCH MIGRATION ATPASE ACTIVITY VIRAL CAPSIDS STRANDED-DNA HELICASE MECHANISM TRANSLOCATION HYDROLYSIS |
| ISSN号 | 1674-1056 |
| 通讯作者 | Xie, P: Chinese Acad Sci, Lab Soft Matter Phys, Inst Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | During the assembly of many viruses, a powerful molecular motor packages the genome into a preassembled capsid. The Bacillus subtilis phage phi 29 is an excellent model system to investigate the DNA packaging mechanism because of its highly efficient in vitro DNA packaging activity and the development of a single-molecule packaging assay. Here we make use of structural and biochemical experimental data to build a physical model of DNA packaging by the phi 29 DNA packaging motor. Based on the model, various dynamic behaviours such as the packaging rate, pause frequency and slip frequency under different ATP concentrations, ADP concentrations, external loads as well as capsid fillings are studied by using Monte Carlo simulation. Good agreement is obtained between the simulated and available experimental results. Moreover, we make testable predictions that should guide future experiments related to motor function. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [10834014, 10674173]; National Basic Research Program of China [2009CB930704] |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/42217]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qian, J,Xie, P,Xue, XG,et al. Modelling of a DNA packaging motor[J]. CHINESE PHYSICS B,2009,18(11):4852. |
| APA | Qian, J,Xie, P,Xue, XG,&Wang, PY.(2009).Modelling of a DNA packaging motor.CHINESE PHYSICS B,18(11),4852. |
| MLA | Qian, J,et al."Modelling of a DNA packaging motor".CHINESE PHYSICS B 18.11(2009):4852. |
入库方式： OAI收割
来源：物理研究所
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