Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease
文献类型：期刊论文
| 作者 | Tian, XS ; Feng, YJ ; Zhao, TZ ; Peng, H ; Yan, JH ; Qi, JX ; Jiang, F ; Tian, KG ; Gao, F |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
 |
| 出版日期 | 2007 |
| 卷号 | 63页码:720 |
| ISSN号 | 1744-3091 |
| 中文摘要 | 3CL protease, a viral chymotrypsin-like proteolytic enzyme, plays a pivotal role in the transcription and replication machinery of many RNA viruses, including porcine reproductive and respiratory syndrome virus (PRRSV). In this study, the full-length 3CL protease from PRRSV was cloned and overexpressed in Escherichia coli. Crystallization experiments yielded crystals that diffracted to 2.1 A resolution and belong to space group C2, with unit-cell parameters a = 112.31, b = 48.34, c = 42.88 A, beta = 109.83 degrees. The Matthews coefficient and the solvent content were calculated to be 2.49 A(3) Da(-1) and 50.61%, respectively, for one molecule in the asymmetric unit. |
| 收录类别 | SCI |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/42252]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Tian, XS,Feng, YJ,Zhao, TZ,et al. Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2007,63:720. |
| APA | Tian, XS.,Feng, YJ.,Zhao, TZ.,Peng, H.,Yan, JH.,...&Gao, F.(2007).Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,63,720. |
| MLA | Tian, XS,et al."Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 63(2007):720. |
入库方式： OAI收割
来源：物理研究所
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