Monitoring the binding of metal cations and histones to DNA in real time using fluorescence assays
文献类型：期刊论文
| 作者 | Liu, YY ; Wang, PY ; Dou, SX ; Zhang, WW ; Wang, XJ ; Sang, HY |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2011 |
| 卷号 | 56期号:11页码:1080 |
| 关键词 | NUCLEIC-ACIDS ANISOTROPY ASSAYS DIVALENT-CATIONS PROTEIN-PROTEIN IONS HELIX CONDENSATION SPECTROSCOPY COMBINATION POLYMERS |
| ISSN号 | 1001-6538 |
| 通讯作者 | Liu, YY: China Agr Univ, Coll Sci, Beijing 100083, Peoples R China. |
| 中文摘要 | The binding of cations (Na(+), K(+), Mg(2+), Ca(2+), Mn(2+)) and histones to DNA can be studied using fluorescence assays. Here, we measured the fluorescence intensity and fluorescence anisotropy of DNA and DNA-histone complexes in the presence of cations. We demonstrate that when different cations are added into a DNA solution, the fluorescence intensities of the stained DNA are reduced by different amounts. Compared with divalent cations, monovalent cations had a weaker effect on fluorescence intensity and fluorescence anisotropy. Divalent (Mn(2+), Mg(2+), Ca(2+)) cations markedly enhanced the fluorescence anisotropy of DNA. The binding modes of monovalent and divalent cations to DNA may be different. Divalent cations can change the structure of DNA molecules, or promote the assembly of DNA strands. The addition of histones causes DNA condensation, which mostly occurs during the first few seconds. Cation binding to DNA is abrupt, and is much faster than that of histones. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [60025516, 10334100]; Scientific Research Startup Foundation of China Agricultural University [2009-2-05] |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/42280]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, YY,Wang, PY,Dou, SX,et al. Monitoring the binding of metal cations and histones to DNA in real time using fluorescence assays[J]. CHINESE SCIENCE BULLETIN,2011,56(11):1080. |
| APA | Liu, YY,Wang, PY,Dou, SX,Zhang, WW,Wang, XJ,&Sang, HY.(2011).Monitoring the binding of metal cations and histones to DNA in real time using fluorescence assays.CHINESE SCIENCE BULLETIN,56(11),1080. |
| MLA | Liu, YY,et al."Monitoring the binding of metal cations and histones to DNA in real time using fluorescence assays".CHINESE SCIENCE BULLETIN 56.11(2011):1080. |
入库方式： OAI收割
来源：物理研究所
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