Mutual Inhibition of RecQ Molecules in DNA Unwinding
文献类型：期刊论文
| 作者 | Pan, BY ; Dou, SX ; Yang, Y ; Xu, YN ; Bugnard, E ; Ding, XY ; Zhang, LY ; Wang, PY ; Li, M ; Xi, XG |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2010 |
| 卷号 | 285期号:21页码:15884 |
| 关键词 | SINGLE-STRANDED-DNA COLI REP HELICASE C VIRUS HELICASE CRYSTAL-STRUCTURES PCRA HELICASE MECHANISM TRANSLOCATION UVRD MONOMER SEPARATION |
| ISSN号 | 0021-9258 |
| 通讯作者 | Dou, SX: Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Helicases make conformational changes and mechanical movements through hydrolysis of NTP to unwind duplex DNA (or RNA). Most helicases require a single-stranded overhang for loading onto the duplex DNA substrates. Some helicases have been observed to exhibit an enhanced unwinding efficiency with increasing length of the single-stranded DNA tail both by preventing reannealing of the unwound DNA and by compensating for premature dissociation of the leading monomers. Here we report a previously unknown mutual inhibition of neighboring monomers in DNA unwinding by the monomeric Escherichia coli RecQ helicase. With single molecule fluorescence resonance energy transfer microscopy, we observed that the unwinding initiation of RecQ at saturating concentrations was more delayed for a long rather than a short tailed DNA. In stopped-flow kinetic studies under both single and multiple turnover conditions, the unwinding efficiency decreased with increasing enzyme concentration for long tailed substrates. In addition, preincubation of RecQ and DNA in the presence of 5'-adenylyl-beta,gamma-imidodiphosphate was observed to alleviate the inhibition. We propose that the mutual inhibition effect results from a forced closure of cleft between the two RecA-like domains of a leading monomer by a trailing one, hence the forward movements of both monomers are stalled by prohibition of ATP binding to the leading one. This effect represents direct evidence for the relative movements of the two RecA-like domains of RecQ in DNA unwinding. It may occur for all superfamily I and II helicases possessing two RecA-like domains. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [10834014, 10674173, 30770517, 30870590]; 973 Project [2009CB930704, 2006CB910302]; Institut Curie and the Centre National de la Recherche Scientifique (CNRS) |
| 语种 | 英语 |
| 公开日期 | 2013-09-18 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/42440]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pan, BY,Dou, SX,Yang, Y,et al. Mutual Inhibition of RecQ Molecules in DNA Unwinding[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2010,285(21):15884. |
| APA | Pan, BY.,Dou, SX.,Yang, Y.,Xu, YN.,Bugnard, E.,...&Xi, XG.(2010).Mutual Inhibition of RecQ Molecules in DNA Unwinding.JOURNAL OF BIOLOGICAL CHEMISTRY,285(21),15884. |
| MLA | Pan, BY,et al."Mutual Inhibition of RecQ Molecules in DNA Unwinding".JOURNAL OF BIOLOGICAL CHEMISTRY 285.21(2010):15884. |
入库方式： OAI收割
来源：物理研究所
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