The zinc-binding motif of human RECQ5 beta suppresses the intrinsic strand-annealing activity of its DExH helicase domain and is essential for the helicase activity of the enzyme
文献类型：期刊论文
| 作者 | Ren, H ; Dou, SX ; Zhang, XD ; Wang, PY ; Kanagaraj, R ; Liu, JI ; Janscak, P ; Hu, JS ; Xi, XG |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2008 |
| 卷号 | 412页码:425 |
| 关键词 | ESCHERICHIA-COLI RECQ DNA HELICASE PROTEIN BLOOMS CLONING DROSOPHILA MECHANISM GENOME FAMILY GENES |
| ISSN号 | 0264-6021 |
| 中文摘要 | RecQ family helicases, functioning as caretakers of genomic integrity, contain a zinc-binding motif which is highly conserved among these helicases, but does not have a substantial structural similarity with any other known zinc-finger folds. In the present study, we show that a truncated variant of the human RECQ5 beta helicase comprised of the conserved helicase domain only, a splice variant named RECQ5 alpha, possesses neither ATPase nor DNA-unwinding activities, but surprisingly displays a strong strand-annealing activity. In contrast, fragments of RECQ5 beta including the intact zinc-binding motif, which is located immediately downstream of the helicase domain, exhibit much reduced strand-annealing activity but are proficient in DNA unwinding. Quantitative measurements indicate that the regulatory role of the zinc-binding motif is achieved by enhancing the DNA-binding affinity of the enzyme. The novel intramolecular modulation of RECQ5 catalytic activity mediated by the zinc-binding motif may represent a universal regulation mode for all RecQ family helicases. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-23 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/45609]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ren, H,Dou, SX,Zhang, XD,et al. The zinc-binding motif of human RECQ5 beta suppresses the intrinsic strand-annealing activity of its DExH helicase domain and is essential for the helicase activity of the enzyme[J]. BIOCHEMICAL JOURNAL,2008,412:425. |
| APA | Ren, H.,Dou, SX.,Zhang, XD.,Wang, PY.,Kanagaraj, R.,...&Xi, XG.(2008).The zinc-binding motif of human RECQ5 beta suppresses the intrinsic strand-annealing activity of its DExH helicase domain and is essential for the helicase activity of the enzyme.BIOCHEMICAL JOURNAL,412,425. |
| MLA | Ren, H,et al."The zinc-binding motif of human RECQ5 beta suppresses the intrinsic strand-annealing activity of its DExH helicase domain and is essential for the helicase activity of the enzyme".BIOCHEMICAL JOURNAL 412(2008):425. |
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来源：物理研究所
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