Thermal-Induced Dissociation and Unfolding of Homodimeric DsbC Revealed by Temperature-Jump Time-Resolved Infrared Spectra
文献类型：期刊论文
| 作者 | Li, H ; Ke, HM ; Ren, GP ; Qiu, XG ; Weng, YX ; Wang, CC |
| 刊名 | BIOPHYSICAL JOURNAL
 |
| 出版日期 | 2009 |
| 卷号 | 97期号:10页码:2811 |
| 关键词 | DISULFIDE BOND FORMATION HELIX-COIL TRANSITION ESCHERICHIA-COLI SECONDARY STRUCTURES PROTEIN-STRUCTURE CRYSTAL-STRUCTURE CYTOCHROME-C FAST EVENTS SPECTROSCOPY ISOMERASE |
| ISSN号 | 0006-3495 |
| 通讯作者 | Weng, YX (reprint author), Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | The thermal stability of DsbC, a homodimeric protein disulfide isomerase in prokaryotic periplasm, has been studied by using temperature-dependent Fourier transformation infrared and time-resolved infrared spectroscopy coupled with temperature-jump initiation. The infrared absorbance thermal titration curves for thermal-induced unfolding of DsbC in D(2)O exhibit a three-state transition with the first transition midpoint temperature at 37.1 +/- 1.1 degrees C corresponding to dissociation, and the second at >74.5 degrees C corresponding to global unfolding and aggregation. The dissociation midpoint temperature of DsbC in phosphate buffer shifts to 49.2 +/- 0.7 degrees C. Temperature-jump time-resolved infrared spectra in D(2)O shows that DsbC dissociates into the corresponding germinate monomeric encounter pair with a time constant of 40 +/- 10 ns independent of the protein concentration and 77% of the newly formed monomeric encounter pair undergoes further coil to helix/loop transition with a time constant of 160 +/- 10 ns. The encounter pair is expected to proceed with further dissociation into monomers. The dissociation of DsbC is confirmed by size-exclusion chromatography and subunit hybridization. The in vivo oxidase activity of DsbC attributed to the monomer has also been observed by using cadmium sensitivity and the oxidative state of beta-lactamase. |
| 收录类别 | SCI |
| 资助信息 | Chinese Ministry of Science and Technology [2006CB910903, 2006CB806508]; China National Science Foundation [30620130109]; Chinese Academy of Sciences Innovative Project [KJCX2-SW-w29] |
| 语种 | 英语 |
| 公开日期 | 2013-09-23 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/45751]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, H,Ke, HM,Ren, GP,et al. Thermal-Induced Dissociation and Unfolding of Homodimeric DsbC Revealed by Temperature-Jump Time-Resolved Infrared Spectra[J]. BIOPHYSICAL JOURNAL,2009,97(10):2811. |
| APA | Li, H,Ke, HM,Ren, GP,Qiu, XG,Weng, YX,&Wang, CC.(2009).Thermal-Induced Dissociation and Unfolding of Homodimeric DsbC Revealed by Temperature-Jump Time-Resolved Infrared Spectra.BIOPHYSICAL JOURNAL,97(10),2811. |
| MLA | Li, H,et al."Thermal-Induced Dissociation and Unfolding of Homodimeric DsbC Revealed by Temperature-Jump Time-Resolved Infrared Spectra".BIOPHYSICAL JOURNAL 97.10(2009):2811. |
入库方式： OAI收割
来源：物理研究所
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