Observation of the in-vivo reporter of green fluorescent protein in a plant root by scanning near-field optical microscopy
文献类型：期刊论文
| 作者 | Sun, JL ; Xu, JH ; Chen, T ; Tan, XJ ; Cao, Y ; Liu, JY ; Xie, AF ; Zhang, ZB ; Guo, JH |
| 刊名 | CHINESE PHYSICS LETTERS
 |
| 出版日期 | 2002 |
| 卷号 | 19期号:9页码:1389 |
| 关键词 | MOLECULES MARKER PROBE CELLS |
| ISSN号 | 0256-307X |
| 通讯作者 | Sun, JL (reprint author), Tsing Hua Univ, Key Lab Atom & Mol Nanosci Educ Minist, Beijing 100084, Peoples R China. |
| 中文摘要 | An in vivo reporter of green fluorescent protein (GFP) in a living plant root has been imaged by scanning near-field optical microscopy (SNOM) in the transmission mode. The exciting light is 488 nm wavelength of the argon ion laser and the bandpass filter (514+/-10) nm is put into the detecting optical pathway. The results indicate that, in the living plant cells, the GFPs gather together and form an area of 2-4 mum, rather than being individually distributed. The transmission coefficient of the eigenfunction is incorporated into Bethe's theoretical model modified by Grober, and the near-field excited light intensity is calculated along the fibre probe axis (z-axis) in the air medium and biological medium. Based on this, along the z-axis direction of the GFP detected in the sample, numerous GFPs locate near the epidermal cells wall (in the range of 0-38 nm) in the living root. The experiments show that SNOM has an advantage of optical nanometre-scale resolution along the z-axis. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/50186]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun, JL,Xu, JH,Chen, T,et al. Observation of the in-vivo reporter of green fluorescent protein in a plant root by scanning near-field optical microscopy[J]. CHINESE PHYSICS LETTERS,2002,19(9):1389. |
| APA | Sun, JL.,Xu, JH.,Chen, T.,Tan, XJ.,Cao, Y.,...&Guo, JH.(2002).Observation of the in-vivo reporter of green fluorescent protein in a plant root by scanning near-field optical microscopy.CHINESE PHYSICS LETTERS,19(9),1389. |
| MLA | Sun, JL,et al."Observation of the in-vivo reporter of green fluorescent protein in a plant root by scanning near-field optical microscopy".CHINESE PHYSICS LETTERS 19.9(2002):1389. |
入库方式： OAI收割
来源：物理研究所
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