Orientation-dependent transparency of metallic interfaces
文献类型：期刊论文
| 作者 | Xu, PX ; Xia, K ; Zwierzycki, M ; Talanana, M ; Kelly, PJ |
| 刊名 | PHYSICAL REVIEW LETTERS
 |
| 出版日期 | 2006 |
| 卷号 | 96期号:17 |
| 关键词 | SPIN POLARIZATION ANDREEV REFLECTION FERROMAGNETS MULTILAYERS |
| ISSN号 | 0031-9007 |
| 通讯作者 | Zwierzycki, M (reprint author), Chinese Acad Sci, Inst Phys, State Key Lab Surface Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | RecQ family helicases play a key role in chromosome maintenance. Despite extensive biochemical, biophysical, and structural studies, the mechanism by which helicase unwinds double-stranded DNA remains to be elucidated. Using a wide array of biochemical and biophysical approaches, we have previously shown that the Escherichia coli RecQ helicase functions as a monomer. In this study, we have further characterized the kinetic mechanism of the RecQ-catalyzed unwinding of duplex DNA using the fluorometric stopped-flow method based on fluorescence resonance energy transfer. Our results show that RecQ helicase binds preferentially to 3'-flanking duplex DNA. Under the pre-steady-state conditions, the burst amplitude reveals a 1: 1 ratio between RecQ and DNA substrate, suggesting that an active monomeric form of RecQ helicase is involved in the catalysis. Under the single-turnover conditions, the RecQ-catalyzed unwinding is independent of the 3'-tail length, indicating that functional interactions between RecQ molecules are not implicated in the DNA unwinding. It was further determined that RecQ unwinds DNA rapidly with a step size of 4 bp and a rate of similar to 21 steps/s. These kinetic results not only further support our previous conclusion that E. coli RecQ functions as a monomer but also suggest that some of the Superfamily 2 helicases may function through an "inchworm" mechanism. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/50465]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xu, PX,Xia, K,Zwierzycki, M,et al. Orientation-dependent transparency of metallic interfaces[J]. PHYSICAL REVIEW LETTERS,2006,96(17). |
| APA | Xu, PX,Xia, K,Zwierzycki, M,Talanana, M,&Kelly, PJ.(2006).Orientation-dependent transparency of metallic interfaces.PHYSICAL REVIEW LETTERS,96(17). |
| MLA | Xu, PX,et al."Orientation-dependent transparency of metallic interfaces".PHYSICAL REVIEW LETTERS 96.17(2006). |
入库方式： OAI收割
来源：物理研究所
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