Penetration and saturation of lysozyme in phospholipid bilayers
文献类型：期刊论文
| 作者 | Yuan, B ; Xing, LL ; Zhang, YD ; Lu, Y ; Luo, YY ; Mai, ZH ; Li, M |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2007 |
| 卷号 | 111期号:22页码:6151 |
| ISSN号 | 1520-6106 |
| 中文摘要 | We show that the combination of X-ray reflectivity, tryptophan fluorescence spectrum, and fluorescence quenching by bromine provides a useful tool to probe the location of lysozyme in lipid bilayers. To this end, we prepare lamellar complexes composed of phospholipids and lysozyme on solid surfaces using a solution-casting method. The proteins lie spontaneously between adjacent bilayers in the complexes. The results indicate that lysozyme may penetrate into the lipid bilayers. But the penetration depth is very shallow, and the tryptophan residues do not penetrate beyond the interface between the hydrocardon core and the headgroup region of the lipid bilayer. The penetration becomes saturated when more proteins are incorporated into the lamellar complex. The excess proteins stay in the interlamellar aqueous layers. |
| 收录类别 | SCI |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/50649]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yuan, B,Xing, LL,Zhang, YD,et al. Penetration and saturation of lysozyme in phospholipid bilayers[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2007,111(22):6151. |
| APA | Yuan, B.,Xing, LL.,Zhang, YD.,Lu, Y.,Luo, YY.,...&Li, M.(2007).Penetration and saturation of lysozyme in phospholipid bilayers.JOURNAL OF PHYSICAL CHEMISTRY B,111(22),6151. |
| MLA | Yuan, B,et al."Penetration and saturation of lysozyme in phospholipid bilayers".JOURNAL OF PHYSICAL CHEMISTRY B 111.22(2007):6151. |
入库方式： OAI收割
来源：物理研究所
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