Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification
文献类型：期刊论文
| 作者 | Jiang, F ; Gu, YX ; Zheng, CD ; Fan, HF |
| 刊名 | CHINESE PHYSICS
 |
| 出版日期 | 2003 |
| 卷号 | 12期号:8页码:890 |
| 关键词 | COMBINING DIRECT-METHODS PROTEIN DATA-BANK ISOMORPHOUS REPLACEMENT DIFFRACTION DATA CRYSTALLOGRAPHY AMBIGUITY CRAMBIN |
| ISSN号 | 1009-1963 |
| 通讯作者 | Jiang, F (reprint author), Chinese Acad Sci, Inst Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | A new method of combining one-wavelength anomalous scattering (OAS) phasing and density modification has been described, in which the improved phases from density modification are re-introduced into OAS phasing. In this way, the phases could be improved iteratively until convergence. The OAS phasing method is based on the previously established sign-probability formula, which breaks the phase ambiguity in the OAS phasing. The implementation of this method has been available in CCP4 as OASIS. This method, although based on direct-methods, could also incorporate known phases and figures of merit into its sign-probability formula. In the implementation of OASIS, the known phases are from the positions of the anomalous scatters. In the current method, the known phases are from the density modification. The current method was tested on phasing a lysozyme crystal using anomalous scattering of sulphur atoms with diffraction data collected on an in-house x-ray source. The resulting map was well connected for the backbone atoms and clearly traceable, with an average map correlation coefficient of 0.6622 for the backbone atoms. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/50880]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Jiang, F,Gu, YX,Zheng, CD,et al. Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification[J]. CHINESE PHYSICS,2003,12(8):890. |
| APA | Jiang, F,Gu, YX,Zheng, CD,&Fan, HF.(2003).Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification.CHINESE PHYSICS,12(8),890. |
| MLA | Jiang, F,et al."Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification".CHINESE PHYSICS 12.8(2003):890. |
入库方式： OAI收割
来源：物理研究所
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