Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method
文献类型：期刊论文
| 作者 | Wang, X ; Lu, H ; Dai, J ; Wen, JA ; Yuan, K ; Lu, HB ; Jin, KJ ; Zhou, YL ; Yang, GZ |
| 刊名 | CHINESE PHYSICS B
 |
| 出版日期 | 2011 |
| 卷号 | 20期号:1 |
| 关键词 | PROTEIN MICROARRAYS GOLD NANOPARTICLES DNA BIOSENSORS |
| ISSN号 | 1674-1056 |
| 通讯作者 | Yang, GZ (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | We successfully conduct the label-free and real-time detection of the interactions between epoxy groups and rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide, and between the hybridization of 5' CTT CAG GTC ATG AGC CTG AT 3' and its complementary 3' GAA GTC CAG TAC TCG GAC TA 5' oligonucleotide, by the oblique-incidence reflectivity difference (OI-RD) method. The dynamic curves of OI-RD signals, corresponding to the kinetic processes of biomolecular combination or hybridization, are acquired. In our case, the combination of epoxy groups with rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide need almost one and a half hours and about two hundred seconds, respectively; and the hybridization of the two oligonucleotides needs about five hundred seconds. The experimental results show that the OI-RD is a promising method for the real-time and label-free detection of biomolecular interactions. |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program in China [2007CB935700] |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/51913]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, X,Lu, H,Dai, J,et al. Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method[J]. CHINESE PHYSICS B,2011,20(1). |
| APA | Wang, X.,Lu, H.,Dai, J.,Wen, JA.,Yuan, K.,...&Yang, GZ.(2011).Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method.CHINESE PHYSICS B,20(1). |
| MLA | Wang, X,et al."Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method".CHINESE PHYSICS B 20.1(2011). |
入库方式： OAI收割
来源：物理研究所
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