SAD phasing by OASIS at different resolutions down to 0.30 nm and below
文献类型：期刊论文
| 作者 | Yao, DQ ; Li, H ; Chen, Q ; Gu, YX ; Zheng, CD ; Lin, ZJ ; Fan, HF ; Watanabe, N ; Sha, BD |
| 刊名 | CHINESE PHYSICS B
 |
| 出版日期 | 2008 |
| 卷号 | 17期号:1页码:1 |
| ISSN号 | 1674-1056 |
| 中文摘要 | Single-wavelength anomalous diffraction (SAD) phasing is increasingly important in solving de novo protein structures. Direct methods have been proved very efficient in SAD phasing. This paper aims at probing the low-resolution limit of direct-method SAD phasing. Two known proteins TT0570 and Tom70p were used as test samples. Sulfur-SAD data of the protein TT0570 were collected with conventional Cu-K alpha source at 0.18 nm resolution. Its truncated subsets respectively at 0.21, 0.30, 0.35 and 0.40 nm resolutions were used in the test. TT0570 Cu-Ka sulfur-SAD data have an expected Bijvoet ratio < Delta> / < F > similar to 0.55%. In the 0.21 nm case, a single run of OASIS-DM-ARP/wARP led automatically to a model containing 1178 of the total 1206 residues all docked into the sequence. In 0.30 and 0.35 nm cases, SAD phasing by OASIS-DM led to traceable electron density maps. In the 0.40 nm case, SAD phasing by OASIS-DM resulted in a degraded electron density map, which may be difficult to trace but still contains useful secondary-structure information. Test on real 0.33 nm selenium-SAD data of the protein Tom70p showed that even automatic model building was not successful, the combination of manual tracing and direct-method fragment extension was capable of significantly improving the electron-density map. This provides the possibility of effectively improving the manually built model before structure refinement is performed. |
| 收录类别 | SCI |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/52287]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yao, DQ,Li, H,Chen, Q,et al. SAD phasing by OASIS at different resolutions down to 0.30 nm and below[J]. CHINESE PHYSICS B,2008,17(1):1. |
| APA | Yao, DQ.,Li, H.,Chen, Q.,Gu, YX.,Zheng, CD.,...&Sha, BD.(2008).SAD phasing by OASIS at different resolutions down to 0.30 nm and below.CHINESE PHYSICS B,17(1),1. |
| MLA | Yao, DQ,et al."SAD phasing by OASIS at different resolutions down to 0.30 nm and below".CHINESE PHYSICS B 17.1(2008):1. |
入库方式： OAI收割
来源：物理研究所
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