Structural changes of purple membrane and bacteriorhodopsin during its denaturation induced by high pH
文献类型：期刊论文
| 作者 | Li, H ; Chen, DL ; Zhong, S ; Xu, B ; Han, BS ; Hu, KS |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2005 |
| 卷号 | 109期号:22页码:11273 |
| 关键词 | ATOMIC-FORCE MICROSCOPY HALOBACTERIUM-HALOBIUM SURFACE-STRUCTURE PROTON PUMP MOLECULE |
| ISSN号 | 1520-6106 |
| 通讯作者 | Hu, KS (reprint author), Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China. |
| 中文摘要 | Bacteriorhodopsin (bR) trimers naturally form two-dimensional hexagonal crystals in purple membrane (PM), which make it very stable. However, the dnaturation of bR was found to occur during a very narrow pH range when the pH was increased above 12.0, as indicated by inactivation of the photochemical cycle observed by flash photolysis kinetic spectra. Here, atomic force microscopy was used to study the surface structural changes of PM during the denaturation process induced by high pH. Together with the absorption and fluorescence spectra, it was found that the structural changes could be divided into three steps. First, some hydrophobic amino acids of bR become exposed to the aqueous environment and PM loses its 2D crystalline structure, transforming into the so-called "nonisland" structure. Second, bR molecules are extracted out of membrane and form protrusions on the surface like islands in the sea; therefore, the "nonisland" structure transforms into the "island" structure. Finally, most bRs break off from the membrane and form large depositions. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/53276]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, H,Chen, DL,Zhong, S,et al. Structural changes of purple membrane and bacteriorhodopsin during its denaturation induced by high pH[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2005,109(22):11273. |
| APA | Li, H,Chen, DL,Zhong, S,Xu, B,Han, BS,&Hu, KS.(2005).Structural changes of purple membrane and bacteriorhodopsin during its denaturation induced by high pH.JOURNAL OF PHYSICAL CHEMISTRY B,109(22),11273. |
| MLA | Li, H,et al."Structural changes of purple membrane and bacteriorhodopsin during its denaturation induced by high pH".JOURNAL OF PHYSICAL CHEMISTRY B 109.22(2005):11273. |
入库方式： OAI收割
来源：物理研究所
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