Structure and Phase Transformation of Oligodeoxynucleotide/Lipid Lipoplexes on Solid Supports
文献类型：期刊论文
| 作者 | Lu, Y ; Hu, SX ; Li, M |
| 刊名 | LANGMUIR
 |
| 出版日期 | 2010 |
| 卷号 | 26期号:5页码:3539 |
| 关键词 | GENE DELIVERY EN SOLUTION DNA COMPLEXES MECHANISM BILAYERS THERAPY SERIES |
| ISSN号 | 0743-7463 |
| 通讯作者 | Li, M (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Lipoplexes are composed of lipids and nucleic acids and have an ordered multilamellar structure with a periodic 1D array of parallel nucleic acid strands in the lipid bilayers. We report a low-angle X-ray diffraction study on solid-supported lipoplex films composed of synthertic single-stranded oligodeoxynucleotides (ssODN) and lipids, The ssODN molecules distribute sparsely in the headgroup regions when the weight concentration of the ssODN in the lipoplex is low. The lipoplex separates into two phases, an ODN-poor phase and an ODN-rich phase, when the weight concentration of the ssODN is increased beyond a level at which the ssODN molecules contact each other so that sonic regions of the lipid bilayers must accommodate two layers of the ssODN. The phase separation is a results of the fusion of such regions to minimize the total interfacial energy of the system. The ssODN molecules distort the lipid bilayers in the ODN-poor phase. The local area per lipid molecule is increased by the distortions so that the interbilayer distance of the lipoplex film is smaller than that of the lipid film without the ssODN. The ODN-rich phase has a much larger interbilayer distance because two layers of ssODN are intercalated into each lipid bilayer. The ssODN molecules are tightly compacted, and the lipid bilayers are not distorted in the ODN-rich phase. |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences [kjcx3-syw-n8]; National Natural Science Foundation of China [10904164, 10674158] |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/53538]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lu, Y,Hu, SX,Li, M. Structure and Phase Transformation of Oligodeoxynucleotide/Lipid Lipoplexes on Solid Supports[J]. LANGMUIR,2010,26(5):3539. |
| APA | Lu, Y,Hu, SX,&Li, M.(2010).Structure and Phase Transformation of Oligodeoxynucleotide/Lipid Lipoplexes on Solid Supports.LANGMUIR,26(5),3539. |
| MLA | Lu, Y,et al."Structure and Phase Transformation of Oligodeoxynucleotide/Lipid Lipoplexes on Solid Supports".LANGMUIR 26.5(2010):3539. |
入库方式： OAI收割
来源：物理研究所
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