Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel
文献类型：期刊论文
| 作者 | Wang, Y ; Huang, YJ ; Wang, JW ; Cheng, C ; Huang, WJ ; Lu, PL ; Xu, YN ; Wang, PY ; Yan, N ; Shi, YG |
| 刊名 | NATURE
 |
| 出版日期 | 2009 |
| 卷号 | 462期号:7272页码:467 |
| 关键词 | ESCHERICHIA-COLI WATER CHANNEL MEMBRANE-PROTEINS NITRATE SELECTIVITY SOFTWARE GLYCEROL NARU IDENTIFICATION RECONSTITUTION |
| ISSN号 | 0028-0836 |
| 通讯作者 | Yan, N (reprint author), Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Struct Biol Ctr, Sch Life Sci, Beijing 100084, Peoples R China. |
| 中文摘要 | FocA is a representative member of the formate-nitrite transporter family, which transports short-chain acids in bacteria, archaea, fungi, algae and parasites. The structure and transport mechanism of the formate-nitrite transporter family remain unknown. Here we report the crystal structure of Escherichia coli FocA at 2.25 angstrom resolution. FocA forms a symmetric pentamer, with each protomer consisting of six transmembrane segments. Despite a lack of sequence homology, the overall structure of the FocA protomer closely resembles that of aquaporin and strongly argues that FocA is a channel, rather than a transporter. Structural analysis identifies potentially important channel residues, defines the channel path and reveals two constriction sites. Unlike aquaporin, FocA is impermeable to water but allows the passage of formate. A structural and biochemical investigation provides mechanistic insights into the channel activity of FocA. |
| 收录类别 | SCI |
| 资助信息 | Ministry of Science and Technology of China [2009CB918801, 2009CB918802]; National Natural Science Foundation of China [30888001]; Beijing Municipal Commissions of Education and Science and Technology; Yuyuan Foundation; Li's Foundation |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/53599]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, Y,Huang, YJ,Wang, JW,et al. Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel[J]. NATURE,2009,462(7272):467. |
| APA | Wang, Y.,Huang, YJ.,Wang, JW.,Cheng, C.,Huang, WJ.,...&Shi, YG.(2009).Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel.NATURE,462(7272),467. |
| MLA | Wang, Y,et al."Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel".NATURE 462.7272(2009):467. |
入库方式： OAI收割
来源：物理研究所
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