Study of cisplatin-induced DNA compaction using single molecule magnetic tweezers
文献类型：期刊论文
| 作者 | Zhang, XH ; Xiao, B ; Hou, XM ; Xu, CH ; Wang, PY ; Li, M |
| 刊名 | ACTA PHYSICA SINICA
 |
| 出版日期 | 2009 |
| 卷号 | 58期号:6页码:4301 |
| 关键词 | ESCHERICHIA-COLI SUPERCOILED DNA KINETICS ELASTICITY BINDING |
| ISSN号 | 1000-3290 |
| 通讯作者 | Li, M (reprint author), Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | We report a single molecule study on cisplatin-induced DNA compaction. It is found that, at a small external tension (< 1 pN), the DNA compaction time course is irregular with many continuous shortening processes and abrupt large size jumps. The time course becomes smooth and hyperbolic when the external tension increases to a moderate value, which is not large enough to inhibit the compaction. The time course depends on the external tension rather than the concentration of cisplatin; the latter only influences the compaction rate. The results are consistent with a looping-and-cross-linking compaction model. Namely, in aqueous solutions, cisplatin forms both monoadducts and diadducts with DNA. When cisplatin induces distant cross-links between DNA bases, micro-loops are formed, which make DNA compact. Further cross-linking between the micro-loops leads to complete compaction of DNA. In addition, the compacted DNA structure is quite stable. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30870590, 10834014] |
| 语种 | 中文 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/53733]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang, XH,Xiao, B,Hou, XM,et al. Study of cisplatin-induced DNA compaction using single molecule magnetic tweezers[J]. ACTA PHYSICA SINICA,2009,58(6):4301. |
| APA | Zhang, XH,Xiao, B,Hou, XM,Xu, CH,Wang, PY,&Li, M.(2009).Study of cisplatin-induced DNA compaction using single molecule magnetic tweezers.ACTA PHYSICA SINICA,58(6),4301. |
| MLA | Zhang, XH,et al."Study of cisplatin-induced DNA compaction using single molecule magnetic tweezers".ACTA PHYSICA SINICA 58.6(2009):4301. |
入库方式： OAI收割
来源：物理研究所
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