Study of the interaction of DNA and histones by spin-stretching and droplet evaporation
文献类型：期刊论文
| 作者 | Liu, YY ; Wang, PY ; Dou, SX ; Zhang, WW ; Wang, XJ ; Sang, HY |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2011 |
| 卷号 | 56期号:12页码:1234 |
| 关键词 | ATOMIC-FORCE MICROSCOPY HIGH-RESOLUTION FLUORESCENCE MICROSCOPY COMBING METHOD COMBED DNA MOLECULES ELASTICITY FIBERS GENOME MOTION |
| ISSN号 | 1001-6538 |
| 通讯作者 | Liu, YY (reprint author), China Agr Univ, Coll Sci, Beijing 100083, Peoples R China. |
| 中文摘要 | Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, DNA-histone complexes are stretched on a hydrophobic polymethyl methacrylate (PMMA) surface and observed directly. We have developed a new method to stretch single DNA-histone complexes, termed spin-stretching. The results show that the histones markedly enhance DNA binding to the PMMA surface. DNA winds around the histones and therefore decreases in length. The number of histones that bind to each DNA molecule is found to correlate with the histone concentration. The combed DNA-histone complexes are found to depend on two factors: the binding force on the surface and the centrifugal force at its local position. Na+ ions should compete with histones for binding to DNA; however, the observed competitive binding effect of Na+ ions at low concentrations was negligible. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [60025516, 10334100]; Scientific Research Start-up Foundation of China Agricultural University [2009-2-05] |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/53800]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, YY,Wang, PY,Dou, SX,et al. Study of the interaction of DNA and histones by spin-stretching and droplet evaporation[J]. CHINESE SCIENCE BULLETIN,2011,56(12):1234. |
| APA | Liu, YY,Wang, PY,Dou, SX,Zhang, WW,Wang, XJ,&Sang, HY.(2011).Study of the interaction of DNA and histones by spin-stretching and droplet evaporation.CHINESE SCIENCE BULLETIN,56(12),1234. |
| MLA | Liu, YY,et al."Study of the interaction of DNA and histones by spin-stretching and droplet evaporation".CHINESE SCIENCE BULLETIN 56.12(2011):1234. |
入库方式： OAI收割
来源：物理研究所
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