Study the effects of divalent metallic ions on the combination of DNA and histones with fluorescence anisotropy assays
文献类型：期刊论文
| 作者 | Liu, YY ; Wang, PY ; Dou, SX ; Xie, P ; Xi, XG |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2007 |
| 卷号 | 52期号:9页码:1166 |
| ISSN号 | 1001-6538 |
| 中文摘要 | The effects of divalent ions (Mn2+, Mg2+, Ca2+) on the interaction between DNA and histone are studied using a fluorescence anisotropy assay. Fluorescence anisotropies of DNA and DNA-histone in the presence of divalent ions (Mn2+, Mg2+, Ca2+) are measured. The results indicate that histone reduces the fluorescence anisotropy of lambda DNA while the divalent ions (Mn2+, Mg2+, Ca2+) significantly enhance the fluorescence anisotropy. Compared to the case of DNA incubated with histone alone, there are more histones binding to DNA when divalent ion, histone and DNA are incubated together. We also find that Mn2+ makes the DNA-histone complexes more condensed than the other ions do. |
| 收录类别 | SCI |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/53880]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, YY,Wang, PY,Dou, SX,et al. Study the effects of divalent metallic ions on the combination of DNA and histones with fluorescence anisotropy assays[J]. CHINESE SCIENCE BULLETIN,2007,52(9):1166. |
| APA | Liu, YY,Wang, PY,Dou, SX,Xie, P,&Xi, XG.(2007).Study the effects of divalent metallic ions on the combination of DNA and histones with fluorescence anisotropy assays.CHINESE SCIENCE BULLETIN,52(9),1166. |
| MLA | Liu, YY,et al."Study the effects of divalent metallic ions on the combination of DNA and histones with fluorescence anisotropy assays".CHINESE SCIENCE BULLETIN 52.9(2007):1166. |
入库方式： OAI收割
来源：物理研究所
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