Study the effects of metallic ions on the combination of DNA and histones with molecular combing technique
文献类型：期刊论文
| 作者 | Liu, YY ; Wang, PY ; Dou, SX ; Xie, P ; Wang, WC ; Yin, HW |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2005 |
| 卷号 | 50期号:8页码:731 |
| 关键词 | NUCLEOSOME FORMATION B-DNA MICROSCOPY CONDENSATION ELASTICITY CATIONS |
| ISSN号 | 1001-6538 |
| 通讯作者 | Wang, PY (reprint author), Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100080, Peoples R China. |
| 中文摘要 | The effects of monovalent (Na+, K+) and divalent (Mg2+, Ca2+, Mn2+) ions on the interaction between DNA and histone are studied using the molecular combing technique. X-DNA molecules and DNA-histone complexes incubated with metal cations (Na+, K+, Mg2+, Ca2+, Mn2+) are stretched on hydrophobic surfaces, and directly observed by fluorescence microscopy. The results indicate that when these cations are added into the DNA solution, the fluorescence intensities of the stained DNA are reduced differently. The monovalent cations (Na+, K+) inhibit binding of histone to DNA. The divalent cations (Mg2+, Ca2+, Mn2+) enhance significantly the binding of histone to DNA and the binding of the DNA-histone complex to the hydrophobic surface. Mn2+ also induces condensation and aggregation of the DNA-histone complex. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/53881]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, YY,Wang, PY,Dou, SX,et al. Study the effects of metallic ions on the combination of DNA and histones with molecular combing technique[J]. CHINESE SCIENCE BULLETIN,2005,50(8):731. |
| APA | Liu, YY,Wang, PY,Dou, SX,Xie, P,Wang, WC,&Yin, HW.(2005).Study the effects of metallic ions on the combination of DNA and histones with molecular combing technique.CHINESE SCIENCE BULLETIN,50(8),731. |
| MLA | Liu, YY,et al."Study the effects of metallic ions on the combination of DNA and histones with molecular combing technique".CHINESE SCIENCE BULLETIN 50.8(2005):731. |
入库方式： OAI收割
来源：物理研究所
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