Studying the interaction between gyrase and DNA using magnetic tweezers
文献类型：期刊论文
| 作者 | Zhang, ZQ ; Liu, YR ; Xie, P ; Li, W ; Dou, SX ; Wang, PY |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2012 |
| 卷号 | 57期号:27页码:3560 |
| ISSN号 | 1001-6538 |
| 关键词 | C-TERMINAL DOMAIN ESCHERICHIA-COLI IIA TOPOISOMERASES BINDING-SITE MECHANISM MOLECULE CLEAVAGE ENZYME RESOLUTION COMPLEXES |
| 通讯作者 | Wang, PY (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | The DNA gyrase of Escherichia coli plays an essential role in the life of this microorganism. It is unique among all topoisomerases because of its ability to introduce negative supercoils into DNA. This study investigated the single molecular interaction of E. coli gyrase with DNA using magnetic tweezers. The results showed that, in the absence of ATP, gyrase weakly binds the G and T segments. The stretched force of 0.7 pN can gradually destroy the binding, whereas that of 5.9 pN directly destroys it. Addition of high concentrations of norfloxacin enhances gyrase binding to both segments, making them adapt to 5.9 pN. DNA gyrase reduces the plectonemic dimension, which was determined by the bacterial enzyme and not by the pull force. Moreover, it has different affinities for positive supercoils, which it prefers, and negative supercoils. The time distribution of the dissociation of gyrase from DNA has a double-exponential form. We herein propose a model to explain this distribution and compare the results with those of other models. |
| 资助信息 | National Natural Science Foundation of China [10834014, 10974248]; National Basic Research Program of China [2009CB930704] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/53883]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang, ZQ,Liu, YR,Xie, P,et al. Studying the interaction between gyrase and DNA using magnetic tweezers[J]. CHINESE SCIENCE BULLETIN,2012,57(27):3560. |
| APA | Zhang, ZQ,Liu, YR,Xie, P,Li, W,Dou, SX,&Wang, PY.(2012).Studying the interaction between gyrase and DNA using magnetic tweezers.CHINESE SCIENCE BULLETIN,57(27),3560. |
| MLA | Zhang, ZQ,et al."Studying the interaction between gyrase and DNA using magnetic tweezers".CHINESE SCIENCE BULLETIN 57.27(2012):3560. |
入库方式： OAI收割
来源：物理研究所
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