A pair of high resolution magnetic tweezers with illumination of total reflection evanescent field and its application in the study of DNA helicases
文献类型：期刊论文
| 作者 | Wang, S ; Zheng, HZ ; Zhao, ZY ; Lu, Y ; Xu, CH |
| 刊名 | ACTA PHYSICA SINICA
 |
| 出版日期 | 2013 |
| 卷号 | 62期号:16 |
| 关键词 | magnetic tweezers total internal reflection fluorescence DNA hairpin helicase |
| ISSN号 | 1000-3290 |
| 通讯作者 | Xu, CH (reprint author), Chinese Acad Sci, Inst Phys, CAS Key Lab Soft Matter Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | The resolution of conventional magnetic tweezers is limited by the Brown motion of magnetic beads. When the force is lower than similar to 10 pN, the resolution of magnetic tweezers decreases significantly because of the increased Brown motion. To improve the resolution of magnetic tweezers under low forces, we combine the total internal reflection fluorescence techniques with magnetic tweezers, and design a novel single molecule connection: "magnetic bead-DNA linker-fluorescent bead-single molecule". With the improved magnetic tweezers, we study the folding dynamics of a DNA hairpin. The results reveal that a nanometer-scale resolution is obtained. By analyzing these results, we calibrate the penetration depth of the total internal reflection field. Finally, we investigate the unwinding dynamics of a BLM helicase core protein. Some preliminary results of the helicase unwinding experiments confirm the practicability of the improved magnetic tweezers in the field of single molecular research. |
| 资助信息 | National Natural Science Foundation of China [61275192, 11004234]; National High Technology Research and Development Program of China [2012AA02A104] |
| 语种 | 中文 |
| 公开日期 | 2014-01-16 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/56733]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, S,Zheng, HZ,Zhao, ZY,et al. A pair of high resolution magnetic tweezers with illumination of total reflection evanescent field and its application in the study of DNA helicases[J]. ACTA PHYSICA SINICA,2013,62(16). |
| APA | Wang, S,Zheng, HZ,Zhao, ZY,Lu, Y,&Xu, CH.(2013).A pair of high resolution magnetic tweezers with illumination of total reflection evanescent field and its application in the study of DNA helicases.ACTA PHYSICA SINICA,62(16). |
| MLA | Wang, S,et al."A pair of high resolution magnetic tweezers with illumination of total reflection evanescent field and its application in the study of DNA helicases".ACTA PHYSICA SINICA 62.16(2013). |
入库方式： OAI收割
来源：物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。