A simple and sensitive fluorescence biosensor for detection of organophosphorus pesticides using H2O2-sensitive quantum dots/bi-enzyme
文献类型：期刊论文
| 作者 | Meng, XW ; Wei, JF ; Ren, XL ; Ren, J ; Tang, FQ |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2013 |
| 卷号 | 47页码:402 |
| 关键词 | Quantum dots Dichlorvos Enzyme biosensor Inhibition Real sample Simple |
| ISSN号 | 0956-5663 |
| 通讯作者 | Ren, J (reprint author), Chinese Acad Sci, Tech Inst Phys & Chem, Lab Controllable Preparat & Applicat Nanomat, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China. |
| 中文摘要 | In this paper, we have developed a simple, fast, convenient and sensitive method for determination of organophosphorus pesticides in real samples based on inhibition mechanism of acetylcholinesterase (AChE). The biosensor is composed of enzymes (AChE and ChOx (choline oxidase)), QDs and acetylcholine (ACh), without any complex process of assembly for biosensor. After the experimental conditions are optimized, the limit of detection (LOD) for dichlorvos (DDVP) is found to be 4.49 nM. Two linear ranges allow a wide determination of DDVP concentration from 4.49 nM to 6780 nM. Furthermore, a possible mechanism is put forward to explain the fluorescence quenching of CdTe QDs in the presence of H2O2. More importantly, the obtained biosensor is proven to be suitable for the detection of residues of organophosphorus pesticides (OPs) in real examples. The excellent performance of this biosensor will facilitate future development of rapid and high-throughput detection of organophosphorus pesticides. (C) 2013 Elsevier B.V. All rights reserved. |
| 资助信息 | National Natural Science Foundation of China [81171454, 61171049, 61178035] |
| 语种 | 英语 |
| 公开日期 | 2014-01-16 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/56745]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Meng, XW,Wei, JF,Ren, XL,et al. A simple and sensitive fluorescence biosensor for detection of organophosphorus pesticides using H2O2-sensitive quantum dots/bi-enzyme[J]. BIOSENSORS & BIOELECTRONICS,2013,47:402. |
| APA | Meng, XW,Wei, JF,Ren, XL,Ren, J,&Tang, FQ.(2013).A simple and sensitive fluorescence biosensor for detection of organophosphorus pesticides using H2O2-sensitive quantum dots/bi-enzyme.BIOSENSORS & BIOELECTRONICS,47,402. |
| MLA | Meng, XW,et al."A simple and sensitive fluorescence biosensor for detection of organophosphorus pesticides using H2O2-sensitive quantum dots/bi-enzyme".BIOSENSORS & BIOELECTRONICS 47(2013):402. |
入库方式： OAI收割
来源：物理研究所
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