Direct Measurement of Sequential Folding Pathway and Energy Landscape of Human Telomeric G-quadruplex Structures
文献类型：期刊论文
| 作者 | Li, W ; Hou, XM ; Wang, PY ; Xi, XG ; Li, M |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2013 |
| 卷号 | 135期号:17页码:6423 |
| ISSN号 | 0002-7863 |
| 通讯作者 | Li, W (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Single-stranded guanine-rich sequences fold into compact G-quadruplexes. Although G-triplexes have been proposed and demonstrated as intermediates in the folding of G-quadruplexes, there is still a.,debate on their folding pathways. In this work, we employed magnetic tweezers to investigate the folding kinetics of single human telomeric G-quadruplexes. in 100 mM Na+ buffer. The results are consistent with a model in which the G-triplex is an in-pathway intermediate in the folding of the G-quadruplex. By finely tuning the force exerted on the G-quadruplex, we observed reversible transitions from the G-quadruplex to the G-triplex as well as from the G-triplex to the unfolded coil when the force was increased from 26 to 39 pN. The. energy. landscape derived from the probability distribution shows clearly that the G-quadruplex goes through an intermediate when it is unfolded; and vice versa. |
| 资助信息 | National Natural Science Foundation of China [11104341, 61275192, 11274374]; 973 Program of China [2013CB837200, 2009CB930704]; 863 Program of China [2012AA02A104]; Northwest AF University [Z101021102, Z111021205] |
| 语种 | 英语 |
| 公开日期 | 2014-01-16 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/56892]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, W,Hou, XM,Wang, PY,et al. Direct Measurement of Sequential Folding Pathway and Energy Landscape of Human Telomeric G-quadruplex Structures[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(17):6423. |
| APA | Li, W,Hou, XM,Wang, PY,Xi, XG,&Li, M.(2013).Direct Measurement of Sequential Folding Pathway and Energy Landscape of Human Telomeric G-quadruplex Structures.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(17),6423. |
| MLA | Li, W,et al."Direct Measurement of Sequential Folding Pathway and Energy Landscape of Human Telomeric G-quadruplex Structures".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.17(2013):6423. |
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来源：物理研究所
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