H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin
文献类型：期刊论文
| 作者 | Chen, P ; Zhao, JC ; Wang, Y ; Wang, M ; Long, HZ ; Liang, D ; Huang, L ; Wen, ZQ ; Li, W ; Li, X ; Feng, HL ; Zhao, HY ; Zhu, P ; Li, M ; Wang, QF ; Li, GH |
| 刊名 | GENES & DEVELOPMENT
 |
| 出版日期 | 2013 |
| 卷号 | 27期号:19页码:2109 |
| 关键词 | chromatin dynamics higher-ordered chromatin structure histone variants H2A.Z H3.3 gene transcription |
| ISSN号 | 0890-9369 |
| 通讯作者 | Li, GH (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China. |
| 中文摘要 | The histone variants H3.3 and H2A.Z have recently emerged as two of the most important features in transcriptional regulation, the molecular mechanism of which still remains poorly understood. In this study, we investigated the regulation of H3.3 and H2A.Z on chromatin dynamics during transcriptional activation. Our in vitro biophysical and biochemical investigation showed that H2A.Z promoted chromatin compaction and repressed transcriptional activity. Surprisingly, with only four to five amino acid differences from the canonical H3, H3.3 greatly impaired higher-ordered chromatin folding and promoted gene activation, although it has no significant effect on the stability of mononucleosomes. We further demonstrated that H3.3 actively marks enhancers and determines the transcriptional potential of retinoid acid (RA)-regulated genes via creating an open chromatin signature that enables the binding of RAR/RXR. Additionally, the H3.3-dependent recruitment of H2A.Z on promoter regions resulted in compaction of chromatin to poise transcription, while RA induction results in the incorporation of H3.3 on promoter regions to activate transcription via counteracting H2A.Z-mediated chromatin compaction. Our results provide key insights into the mechanism of how histone variants H3.3 and H2A.Z function together to regulate gene transcription via the modulation of chromatin dynamics over the enhancer and promoter regions. |
| 资助信息 | Ministry of Science and Technology [2011CB966303, 2009CB825503]; Chinese Academy of Sciences (CAS) Strategic Priority Research Program [XDA01010304, XDA01010305]; Key Research Program [KSZD-EW-Z-009]; National Natural Science Foundation of China [91019007, 31071147, 91219202, 31000566, 61275192]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry |
| 语种 | 英语 |
| 公开日期 | 2014-01-16 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/57062]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen, P,Zhao, JC,Wang, Y,et al. H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin[J]. GENES & DEVELOPMENT,2013,27(19):2109. |
| APA | Chen, P.,Zhao, JC.,Wang, Y.,Wang, M.,Long, HZ.,...&Li, GH.(2013).H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin.GENES & DEVELOPMENT,27(19),2109. |
| MLA | Chen, P,et al."H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin".GENES & DEVELOPMENT 27.19(2013):2109. |
入库方式： OAI收割
来源：物理研究所
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