Quantum dots-based fluorescent probes for turn-on and turn-off sensing of butyrylcholinesterase
文献类型：期刊论文
| 作者 | Chen, ZZ ; Ren, XL ; Meng, XW ; Tan, LF ; Chen, D ; Tang, FQ |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2013 |
| 卷号 | 44页码:204 |
| 关键词 | Quantum dots Butyrylcholinesterase Fluorescence Detection Turn-on Turn-off |
| ISSN号 | 0956-5663 |
| 通讯作者 | Tang, FQ (reprint author), Chinese Acad Sci, Tech Inst Phys & Chem, Lab Controllable Preparat & Applicat Nanomat, 29 Zhongguancun East Rd, Beijing 100190, Peoples R China. |
| 中文摘要 | A novel turn-on and turn-off sensor based on the fluorescence change of quantum dots (QDs) has been developed to detect the activity of butyrylcholinesterase (BChE). In the turn-on sensing system, we realized the detection of BChE with just one enzyme. A linear calibration plot of the activity of BChE was obtained in the wide amounts range from 10 to 1000 U/L and the detection limit was 10 U/L. In the turn-off sensing system, we realized the sensing of BChE with a wide linear relationship of 10-2000 U/L which was much wider than many other detection methods. We also studied the application in serum sample detection. The BChE sensor shows great performances. Furthermore, the turn-on and turn-off sensing mechanisms were studied extensively. These results showed that our strategy would most probably be applicable in assembling diagnostic micro-device for realizing the rapid clinical analysis of BChE. (c) 2013 Elsevier B.V. All rights reserved. |
| 资助信息 | National Natural Science Foundation of China [81171454, 61178035, 61171049]; State Key Laboratory of Bioelectronics of Southeast University |
| 语种 | 英语 |
| 公开日期 | 2014-01-16 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/57370]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen, ZZ,Ren, XL,Meng, XW,et al. Quantum dots-based fluorescent probes for turn-on and turn-off sensing of butyrylcholinesterase[J]. BIOSENSORS & BIOELECTRONICS,2013,44:204. |
| APA | Chen, ZZ,Ren, XL,Meng, XW,Tan, LF,Chen, D,&Tang, FQ.(2013).Quantum dots-based fluorescent probes for turn-on and turn-off sensing of butyrylcholinesterase.BIOSENSORS & BIOELECTRONICS,44,204. |
| MLA | Chen, ZZ,et al."Quantum dots-based fluorescent probes for turn-on and turn-off sensing of butyrylcholinesterase".BIOSENSORS & BIOELECTRONICS 44(2013):204. |
入库方式： OAI收割
来源：物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。