Transplatin enhances effect of cisplatin on both single DNA molecules and live tumor cells
文献类型：期刊论文
| 作者 | Liu, YR ; Ji, C ; Zhang, HY ; Dou, SX ; Xie, P ; Wang, WC ; Wang, PY |
| 刊名 | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2013 |
| 卷号 | 536期号:1页码:12 |
| 关键词 | Transplatin Cisplatin Kinked-loop structure Single DNA molecule Tumor cell Cytotoxicity |
| ISSN号 | 0003-9861 |
| 通讯作者 | Wang, PY (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Cisplatin is the main platinum antitumor drug applied in clinical settings. However, its trans isomer, transplatin, is known to have an ineffective antitumor activity. Despite intensive studies in this field, the structural and biophysical properties of DNA molecules reacting with these two platinum complexes have not been fully elucidated. In the present study, we observed that transplatin made efficient cross-linking of DNA in the vicinity of cisplatin adducts. High-resolution atomic force microscopy studies revealed that the transplatin-induced cross-linkings of nucleotides flanking cisplatin adducts were characterized by kinked-loop structures with rod-like shapes of nanometer scales (similar to 10-60 nm). The results were further confirmed by denaturing gel electrophoresis and single-molecule experiment using magnetic tweezers. In vivo studies revealed that transplatin and cisplatin co-treatment could induce a considerable amount of kinked loops with smaller sizes (similar to 15 nm) in cellular DNA. Furthermore, compared with cisplatin treatment alone, the co-treatment resulted in enhanced cytotoxicity, increased amount of interstrand cross-links, and cell lesions more reluctant to cellular repair system. The results of the present study provide a new clue for understanding the stepwise reactions of DNA with platinum drugs and might serve as a basis for the development of a new antitumor strategy. (C) 2013 Elsevier Inc. All rights reserved. |
| 资助信息 | National Natural Science Foundation of China [11274374, 11204363, 31070751]; National Basic Research Program of China (973 Program) [2009CB930704, 2013CB837200] |
| 语种 | 英语 |
| 公开日期 | 2014-01-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/57621]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, YR,Ji, C,Zhang, HY,et al. Transplatin enhances effect of cisplatin on both single DNA molecules and live tumor cells[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2013,536(1):12. |
| APA | Liu, YR.,Ji, C.,Zhang, HY.,Dou, SX.,Xie, P.,...&Wang, PY.(2013).Transplatin enhances effect of cisplatin on both single DNA molecules and live tumor cells.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,536(1),12. |
| MLA | Liu, YR,et al."Transplatin enhances effect of cisplatin on both single DNA molecules and live tumor cells".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 536.1(2013):12. |
入库方式： OAI收割
来源：物理研究所
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