Effect of Different Bacterial-Feeding Nematode Species on Soil Bacterial Numbers, Activity, and Community Composition
文献类型:期刊论文
作者 | Hai-Feng XIAO ; Gen LI ; Da-Ming LI ; Feng HU ; Hui-Xin LI |
刊名 | Pedosphere
![]() |
出版日期 | 2014 |
卷号 | 24期号:1页码:116-124 |
关键词 | CARD-FISH CO2 evolution DGGE gene sequence gram-negative bacteria |
中文摘要 | The effects of bacterial-feeding nematodes on bacterial number, activity, and community composition were studied through a microcosm experiment using sterilized soil inoculated with soil bacteria (soil suspension) and with bacteria and three species of bacterial-feeding nematodes (Cephalobus persegnis, Protorhabditis filiformis, and Caenorhabditis elegans). Catalyzed reporter deposition-fluorescence in situ hybridization, CO2 evolution, and denaturing gradient gel electrophoresis (DGGE) of PCR amplified 16S rRNA gene fragments were used to investigate bacterial numbers, activity, and community composition, respectively. Our results showed that bacterial numbers and activity significantly increased in the presence of bacterial-feeding nematodes, which indicated that bacterial-feeding nematodes had a significant positive effect on soil bacteria. The different nematode species had different effects on bacterial numbers and activity. C. persegnis and P. filiformis, isolated from native soil, increased the bacterial number and activity more than C. elegans. The DGGE analysis results showed that dominant bacterial species significantly differed among the treatments, which suggested that bacterial-feeding nematode species modified the bacterial community composition in soil. Further gene sequence analysis results showed that the dominant bacterial species in this study were gram-negative bacteria. Given the completely same conditions except nematode species, the varied selective feeding behavior of different nematode species was the most likely reason for the altered bacterial community composition. Overall, the alteration of bacterial numbers, activity and community composition resulting from the bacterial-feeding nematodes may ultimately affect soil ecological functioning and processes. |
公开日期 | 2014-01-06 |
源URL | [http://ir.xtbg.org.cn/handle/353005/4815] ![]() |
专题 | 西双版纳热带植物园_土壤生态组 |
推荐引用方式 GB/T 7714 | Hai-Feng XIAO,Gen LI,Da-Ming LI,et al. Effect of Different Bacterial-Feeding Nematode Species on Soil Bacterial Numbers, Activity, and Community Composition[J]. Pedosphere,2014,24(1):116-124. |
APA | Hai-Feng XIAO,Gen LI,Da-Ming LI,Feng HU,&Hui-Xin LI.(2014).Effect of Different Bacterial-Feeding Nematode Species on Soil Bacterial Numbers, Activity, and Community Composition.Pedosphere,24(1),116-124. |
MLA | Hai-Feng XIAO,et al."Effect of Different Bacterial-Feeding Nematode Species on Soil Bacterial Numbers, Activity, and Community Composition".Pedosphere 24.1(2014):116-124. |
入库方式: OAI收割
来源:西双版纳热带植物园
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。