中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
The Regulatory Role of the Adrenergic Agonists Phenylephrine and Isoproterenol on Fetal Hemoglobin Expression and Erythroid Differentiation

文献类型:期刊论文

作者Mei, Yun1,2; Yin, Naida1; Jin, Xia1; He, Jiangyan1; Yin, Zhan1
刊名ENDOCRINOLOGY
出版日期2013-12-01
卷号154期号:12页码:4640-4649
关键词GLOBIN MESSENGER-RNA GENE-EXPRESSION K562 CELLS ACTIVATION ZEBRAFISH INDUCTION STIMULATION PROGENITORS PATHWAYS INDUCERS
ISSN号0013-7227
通讯作者Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Dept Environm Biol & Human Hlth, 7 South Donghu St, Wuhan 430072, Huibei, Peoples R China.
中文摘要It has been reported that various endocrine hormones exert prominent effects on erythropoiesis. We conducted experiments to identify the mechanisms involved in the regulatory functions of adrenergic signaling on erythroid differentiation and the expression of hemoglobin genes. The reactivation of fetal hemoglobin (HbF) expression is also an important therapeutic option in patients with hemoglobin disorders. We determined that the adrenergic agonists phenylephrine (PE) and isoproterenol (ISO) can induce the production of beta-hemoglobin embryonic 1 (hbbe1) mRNA and protein in adult zebrafish erythrocytes. Elevated levels of HbF mRNA and protein were also observed in human K562 cells after the adrenergic agonist treatments. In addition, elevated levels of histone acetylation were observed in both the PE-and the ISO-treated K562 cells. Additionally, our data further indicate that the induction effects of the adrenergic agonists on HbF synthesis and erythroid differentiation in K562 cells are mainly mediated by the p38 MAPK/cAMP response element binding pathway. In summary, the present study identifies the role of the adrenergic agonists PE and ISO on p38 MAPK and ERK signaling for the stimulation of HbF production and erythroid differentiation.
英文摘要It has been reported that various endocrine hormones exert prominent effects on erythropoiesis. We conducted experiments to identify the mechanisms involved in the regulatory functions of adrenergic signaling on erythroid differentiation and the expression of hemoglobin genes. The reactivation of fetal hemoglobin (HbF) expression is also an important therapeutic option in patients with hemoglobin disorders. We determined that the adrenergic agonists phenylephrine (PE) and isoproterenol (ISO) can induce the production of beta-hemoglobin embryonic 1 (hbbe1) mRNA and protein in adult zebrafish erythrocytes. Elevated levels of HbF mRNA and protein were also observed in human K562 cells after the adrenergic agonist treatments. In addition, elevated levels of histone acetylation were observed in both the PE-and the ISO-treated K562 cells. Additionally, our data further indicate that the induction effects of the adrenergic agonists on HbF synthesis and erythroid differentiation in K562 cells are mainly mediated by the p38 MAPK/cAMP response element binding pathway. In summary, the present study identifies the role of the adrenergic agonists PE and ISO on p38 MAPK and ERK signaling for the stimulation of HbF production and erythroid differentiation.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Endocrinology & Metabolism
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]GLOBIN MESSENGER-RNA ; GENE-EXPRESSION ; K562 CELLS ; ACTIVATION ; ZEBRAFISH ; INDUCTION ; STIMULATION ; PROGENITORS ; PATHWAYS ; INDUCERS
收录类别SCI
资助信息National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]
语种英语
WOS记录号WOS:000327545200020
公开日期2014-01-24
源URL[http://ir.ihb.ac.cn/handle/342005/19784]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Huibei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Mei, Yun,Yin, Naida,Jin, Xia,et al. The Regulatory Role of the Adrenergic Agonists Phenylephrine and Isoproterenol on Fetal Hemoglobin Expression and Erythroid Differentiation[J]. ENDOCRINOLOGY,2013,154(12):4640-4649.
APA Mei, Yun,Yin, Naida,Jin, Xia,He, Jiangyan,&Yin, Zhan.(2013).The Regulatory Role of the Adrenergic Agonists Phenylephrine and Isoproterenol on Fetal Hemoglobin Expression and Erythroid Differentiation.ENDOCRINOLOGY,154(12),4640-4649.
MLA Mei, Yun,et al."The Regulatory Role of the Adrenergic Agonists Phenylephrine and Isoproterenol on Fetal Hemoglobin Expression and Erythroid Differentiation".ENDOCRINOLOGY 154.12(2013):4640-4649.

入库方式: OAI收割

来源:水生生物研究所

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