Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos
文献类型：期刊论文
| 作者 | Yue, Hua-Mei1,2; Li, Zhi1; Wu, Nan1; Liu, Zhen1; Wang, Yang1; Gui, Jian-Fang1 |
| 刊名 | BIOLOGY OF REPRODUCTION
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 89期号:4 |
| 关键词 | cell synchrony early cleavage histone variant midblastula transition oocyte-specific histone |
| ISSN号 | 0006-3363 |
| 通讯作者 | Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Oocyte-specific histone variants have been expected to play significant roles in early embryonic development, but the exact evidence and the biological function have remained unclear. Here, we present evidence that H2af1o, an oocyte-specific H2A variant, is required for cell synchrony before midblastula transition in early zebrafish embryos. The H2A variant is oocyte specific, peaks in mature eggs, and is supplied to early embryos. We constructed a series of deletion plasmids of the zebrafish h2af1o tagged with EGFP and determined the main key function regions including nuclear localization signal of N-terminal 25 amino acids and nucleosome binding region of 110-122 amino acid sequence in the C-terminus by microinjecting them into one-cell-stage zebrafish embryos. In comparison with ubiquitous H2A. X, the H2af1o was revealed to confer a more open structure than canonical H2A in the nucleosomes. Furthermore, we conducted the h2af1o-specific morpholino knockdown analysis in early embryos of zebrafish and revealed its biological function for maintaining cell synchrony division because the H2af1o deficiency disturbed cell synchrony in early cleavages before midblastula transition. Therefore, our current findings provided the first case to understand the biological function of maternal oocyte-specific histone variants in vertebrates. |
| 英文摘要 | Oocyte-specific histone variants have been expected to play significant roles in early embryonic development, but the exact evidence and the biological function have remained unclear. Here, we present evidence that H2af1o, an oocyte-specific H2A variant, is required for cell synchrony before midblastula transition in early zebrafish embryos. The H2A variant is oocyte specific, peaks in mature eggs, and is supplied to early embryos. We constructed a series of deletion plasmids of the zebrafish h2af1o tagged with EGFP and determined the main key function regions including nuclear localization signal of N-terminal 25 amino acids and nucleosome binding region of 110-122 amino acid sequence in the C-terminus by microinjecting them into one-cell-stage zebrafish embryos. In comparison with ubiquitous H2A. X, the H2af1o was revealed to confer a more open structure than canonical H2A in the nucleosomes. Furthermore, we conducted the h2af1o-specific morpholino knockdown analysis in early embryos of zebrafish and revealed its biological function for maintaining cell synchrony division because the H2af1o deficiency disturbed cell synchrony in early cleavages before midblastula transition. Therefore, our current findings provided the first case to understand the biological function of maternal oocyte-specific histone variants in vertebrates. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Reproductive Biology |
| 研究领域[WOS] | Reproductive Biology |
| 关键词[WOS] | CARASSIUS-AURATUS-GIBELIO ; HISTONE VARIANTS ; LINKER HISTONE ; CLONAL DIVERSITY ; NUCLEOSOME ; CHROMATIN ; EXPRESSION ; BINDING ; EMBRYOGENESIS ; RESOLUTION |
| 收录类别 | SCI |
| 资助信息 | National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] |
| 语种 | 英语 |
| WOS记录号 | WOS:000326925800001 |
| 公开日期 | 2014-01-24 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19774]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Hua-Mei,Li, Zhi,Wu, Nan,et al. Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos[J]. BIOLOGY OF REPRODUCTION,2013,89(4). |
| APA | Yue, Hua-Mei,Li, Zhi,Wu, Nan,Liu, Zhen,Wang, Yang,&Gui, Jian-Fang.(2013).Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos.BIOLOGY OF REPRODUCTION,89(4). |
| MLA | Yue, Hua-Mei,et al."Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos".BIOLOGY OF REPRODUCTION 89.4(2013). |
入库方式： OAI收割
来源：水生生物研究所
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