cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides
文献类型：期刊论文
| 作者 | Citarasu, Thavasimuthu1,2; Zhou, Li2; Yang, Wang2; Gui, Jian Fang2 |
| 刊名 | INDIAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 12期号:3页码:330-335 |
| 关键词 | Acyl-Co A binding protein (ACBP) cDNA cloning Epinephelus coioides |
| ISSN号 | 0972-5849 |
| 通讯作者 | Citarasu, T (reprint author), Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India. |
| 中文摘要 | cDNA of acyl-CoA binding protein (ACBP) was constructed, cloned and expressed in hypothalamus and pituitary of orange-spotted grouper, Epinephelus coioides. The ACBP gene contains 630 nucleotides including the open reading frame (ORE) of length 128 bp. The open reading frame was started with ATG codon at the position 462 and terminated with a stop codon TAA at the position 600. The ORE sequence consisted of 42 amino acids and the calculated mol wt of the protein was approx 4.62 kDa (GenBank: FJ939282.1). The highest identity was observed with the puffer fish, Tetraodon nigroviridis and Anoplopoma fimbria with homology of 83 and 88.1%, respectively. RT-PCR results revealed that ACBP mRNA was abundantly expressed in pituitary, hypothalamus and medulla oblongata. Further, ACBP was cloned in pGEX-KG expression vector and transformed into BL21(DE3). The protein was purified by affinity chromatography and partially characterized. The abundant expression of ACBP in pituitary and hypothalamus, in the present study, indicated that it might be involved in reproduction. |
| 英文摘要 | cDNA of acyl-CoA binding protein (ACBP) was constructed, cloned and expressed in hypothalamus and pituitary of orange-spotted grouper, Epinephelus coioides. The ACBP gene contains 630 nucleotides including the open reading frame (ORE) of length 128 bp. The open reading frame was started with ATG codon at the position 462 and terminated with a stop codon TAA at the position 600. The ORE sequence consisted of 42 amino acids and the calculated mol wt of the protein was approx 4.62 kDa (GenBank: FJ939282.1). The highest identity was observed with the puffer fish, Tetraodon nigroviridis and Anoplopoma fimbria with homology of 83 and 88.1%, respectively. RT-PCR results revealed that ACBP mRNA was abundantly expressed in pituitary, hypothalamus and medulla oblongata. Further, ACBP was cloned in pGEX-KG expression vector and transformed into BL21(DE3). The protein was purified by affinity chromatography and partially characterized. The abundant expression of ACBP in pituitary and hypothalamus, in the present study, indicated that it might be involved in reproduction. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RAT-BRAIN ; INHIBITOR ; ENDOZEPINE ; COENZYME ; DBI |
| 收录类别 | SCI |
| 资助信息 | CAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy |
| 语种 | 英语 |
| WOS记录号 | WOS:000327558100004 |
| 公开日期 | 2014-01-24 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19778]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Citarasu, Thavasimuthu,Zhou, Li,Yang, Wang,et al. cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides[J]. INDIAN JOURNAL OF BIOTECHNOLOGY,2013,12(3):330-335. |
| APA | Citarasu, Thavasimuthu,Zhou, Li,Yang, Wang,&Gui, Jian Fang.(2013).cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides.INDIAN JOURNAL OF BIOTECHNOLOGY,12(3),330-335. |
| MLA | Citarasu, Thavasimuthu,et al."cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides".INDIAN JOURNAL OF BIOTECHNOLOGY 12.3(2013):330-335. |
入库方式： OAI收割
来源：水生生物研究所
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