Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor
文献类型：期刊论文
| 作者 | Liu L(刘丽) ; Viallat A; Jin G(靳刚)
|
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2014-01 |
| 卷号 | 190页码:221-226 |
| 通讯作者邮箱 | annie.viallat@inserm.fr; gajin@imech.ac.cn |
| 关键词 | Giant lipid vesicles (GUVs) Vesicles adhesion Non-specific adhesion Total internal reflection imaging ellipsometry (TIRIE) Biosensor |
| ISSN号 | 0925-4005 |
| 产权排序 | [Liu, Li; Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China; [Viallat, Annie] Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France |
| 通讯作者 | Viallat, A (reprint author), Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France. |
| 合作状况 | 国际 |
| 中文摘要 | The behavior of giant lipid vesicles (GUVs) interacting with an adhesive surface coated with poly-L-lysine is investigated by a biosensor based on total internal reflection imaging ellipsometry (TIRIE). The adhesion of GUVs on the adhesive surface is regulated by the concentration of the poly-L-lysine coating, the pH of the vesicle suspension, and the flow rate of the suspending fluid. The TIRIE biosensor detects very clearly and sensitively GUV adsorption, flattening, rupture, and de-adhesion, all these behaviors being independently observed by phase contrast microscopy. These results show that the setting of the TIRIE biosensor, optimized for molecular sensing can be extended to GUV sensing. This work paves the way to develop a new sensitive high-throughput, low-consumption and low-cost biosensor technology for micron-size objects such as cells, capsules and liposomes. |
| 学科主题 | 交叉与边缘领域的力学 |
| 分类号 | 二类/Q1 |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1016/j.snb.2013.08.044 |
| 语种 | 英语 |
| WOS记录号 | WOS:000326687700029 |
| 公开日期 | 2014-02-13 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/48076]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Liu L,Viallat A,Jin G. Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,190:221-226. |
| APA | 刘丽,Viallat A,&靳刚.(2014).Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor.SENSORS AND ACTUATORS B-CHEMICAL,190,221-226. |
| MLA | 刘丽,et al."Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor".SENSORS AND ACTUATORS B-CHEMICAL 190(2014):221-226. |
入库方式： OAI收割
来源：力学研究所
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